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anti-Mouse (Murine) RHEB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RHEB Antibody (supplied by: Log in to see )

Antigen
Ras Homolog Enriched in Brain (RHEB) Antibodies
  • im:7161941
  • RHEB
  • rheb2
  • RHEB2
  • zgc:153231
Epitope
Center
13
9
9
8
8
8
6
3
3
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
73
53
1
1
1
1
Host
Rabbit
88
45
Clonality
Polyclonal
Conjugate
This RHEB antibody is un-conjugated
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
111
32
26
15
11
7
5
2
1
Supplier
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Catalog No. ABIN500597
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN303237 IHC (p) WB Rabbit Middle Region Log in to see Polyclonal
0.0008982679 ABIN704771 IF (p) IHC (p) Rabbit IgG AA 100-140 Log in to see Polyclonal
0.0008982679 ABIN605028 FACS IHC (p) WB Rabbit IgG Asn50 Log in to see Polyclonal
0.0008982679 ABIN1738859 IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN704773 IHC (p) Biotin Rabbit IgG AA 100-140 Log in to see Polyclonal
0.0008982679 ABIN704780 IHC (p) HRP Rabbit IgG AA 100-140 Log in to see Polyclonal
0.0008982679 ABIN2964045 IHC (p) ELISA WB Mouse IgG1 kappa Log in to see 2C11
0.0008982679 ABIN4350383 ELISA IF IHC IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-RHEB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-RHEB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 9 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Western Blotting (WB) 36 Antibodies 56 Antibodies

General

Antigen Ras Homolog Enriched in Brain (RHEB) Antibodies
Epitope Center
(13), (9), (9), (8), (8), (8), (6), (3), (3), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (73), (53), (1), (1), (1), (1)
Host Rabbit
(88), (45)
Clonality
Polyclonal
Conjugate This RHEB antibody is un-conjugated
(3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(111), (32), (26), (15), (11), (7), (5), (2), (1)
Pubmed 4 references available
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Product Details anti-RHEB Antibody

Target Details RHEB Application Details Handling References for anti-RHEB Antibody (ABIN500597) Images
Specificity This antibody detects Rheb at center.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen Rheb antibody was raised against a 15 amino acid peptide from the middle region of human Rheb.
Isotype IgG

Target Details RHEB

Product Details anti-RHEB Antibody Application Details Handling References for anti-RHEB Antibody (ABIN500597) Images back to top
Antigen
Alternative Name RHEB (RHEB Antibody Abstract)
Background Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway (3-5). TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2 (3).Synonyms: GTP-binding protein Rheb, RHEB2, Ras homolog enriched in brain
Gene ID 6009
UniProt Q15382
Research Area Cancer, Transcription Factors
Pathways RTK Signaling

Application Details

Product Details anti-RHEB Antibody Target Details RHEB Handling References for anti-RHEB Antibody (ABIN500597) Images back to top
Application Notes ELISA. Western blot: 1 μg/mL. Immunohistochemistry on paraffin sections
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RHEB Antibody Target Details RHEB Application Details References for anti-RHEB Antibody (ABIN500597) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-RHEB Antibody (ABIN500597)

Product Details anti-RHEB Antibody Target Details RHEB Application Details Handling Images back to top
Background publications

Stocker, Radimerski, Schindelholz, Wittwer, Belawat, Daram, Breuer, Thomas, Hafen: "Rheb is an essential regulator of S6K in controlling cell growth in Drosophila." in: Nature cell biology, Vol. 5, Issue 6, pp. 559-65, 2003

Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003

Yee, Worley: "Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 921-33, 1997

Yamagata, Sanders, Kaufmann, Yee, Barnes, Nathans, Worley: "rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein." in: The Journal of biological chemistry, Vol. 269, Issue 23, pp. 16333-9, 1994

Images

Product Details anti-RHEB Antibody Target Details RHEB Application Details Handling References for anti-RHEB Antibody (ABIN500597) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RHEB antibody (Ras Homolog Enriched in Brain) (Center) (ABIN500597) Immunohistochemistry of Rheb in mouse brain tissue with this product at 2 μg/ml.
Western Blotting (WB) image for anti-RHEB antibody (Ras Homolog Enriched in Brain) (Center) (ABIN500597) Western blot analysis of Rheb in mouse brain cell lysate with this product at (A) 1, ...