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anti-Human RYK antibody for Western Blotting

Recommended RYK Antibody (supplied by: Log in to see )

Antigen
RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
  • AW536699
  • D3S3195
  • d3s3195
  • ERK-3
  • JTK5
  • jtk5
  • JTK5A
  • jtk5a
  • MGC114645
  • RYK
  • ryk1
  • RYK1
  • Vik
  • wu:fb37b12
  • wu:fi05b06
  • zgc:158381
Epitope
Middle Region
19
17
15
14
14
11
8
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
93
20
9
1
1
1
Host
Rabbit
66
23
10
3
Clonality
Polyclonal
Conjugate
This RYK antibody is un-conjugated
9
9
8
8
8
8
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
90
68
31
7
3
2
2
1
1
Supplier
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Catalog No. ABIN453358
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398072 ABIN392051 IHC (p) WB Rabbit Ig AA 561-591, C-Term Log in to see Polyclonal 1
0.13398072 ABIN392050 IHC (p) WB Rabbit Ig AA 160-190 Log in to see Polyclonal 2
0.13398072 ABIN264516 WB Mouse IgG1 Log in to see F35 P7 D7 F5 1
0.13398072 ABIN392052 WB Rabbit Ig AA 383-410, Center, Cys397 Log in to see Polyclonal
0.13398072 ABIN659140 WB Mouse IgG2b, kappa Log in to see 240CT2-2-4
0.13398072 ABIN659141 WB Mouse IgG2b, kappa Log in to see 240CT2-2-4
0.13398072 ABIN1538306 WB Rabbit Ig AA 321-348, Center Log in to see Polyclonal
0.13398072 ABIN2501804 WB Mouse IgG2b, kappa Log in to see 240CT2-2-4
0.13398072 ABIN2501803 WB Mouse IgG2b, kappa Log in to see 240CT2-2-4
0.13398072 ABIN2501808 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13398072 ABIN2501805 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13398072 ABIN2501807 IHC WB Rabbit Ig Fraction Log in to see Polyclonal
0.13398072 ABIN1327384 WB Mouse AA 1-610, full length Log in to see Polyclonal
0.13398072 ABIN307128 WB Mouse IgG1 Log in to see F35 P7 D7 F5 1
0.13398072 ABIN2501806 ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.0011731815 ABIN359937 EIA IHC (p) WB Rabbit Ig AA 150-200 Log in to see Polyclonal 5
0.0011731815 ABIN359938 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 1
0.0011731815 ABIN1500782 WB Mouse IgG2b, kappa Log in to see
0.0011731815 ABIN1932418 IHC ELISA WB Biotin Rabbit IgG AA 168-198 Log in to see Polyclonal
0.0011731815 ABIN1932417 IHC ELISA WB APC Rabbit IgG AA 168-198 Log in to see Polyclonal

Top referenced anti-RYK antibody for Western Blotting

Similar anti-RYK Antibodies

Application / Reactivity Human
ELISA 62 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Western Blotting (WB) 85 Antibodies

General

Antigen RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
Epitope Middle Region
(19), (17), (15), (14), (14), (11), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(93), (20), (9), (1), (1), (1)
Host Rabbit
(66), (23), (10), (3)
Clonality
Polyclonal
Conjugate This RYK antibody is un-conjugated
(9), (9), (8), (8), (8), (8)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(90), (68), (31), (7), (3), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-RYK Antibody

Target Details RYK Application Details Handling References for anti-RYK Antibody (ABIN453358) Images
Specificity This antibody reacts to Tyrosine-protein kinase RYK.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the Center region of human RYK

Target Details RYK

Product Details anti-RYK Antibody Application Details Handling References for anti-RYK Antibody (ABIN453358) Images back to top
Antigen
Alternative Name Tyrosine-Protein Kinase RYK (RYK Antibody Abstract)
Background RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.Synonyms: JTK5A
Gene ID 6259
NCBI Accession NP_001005861
UniProt P34925
Research Area Kinases/Phosphatases
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-RYK Antibody Target Details RYK Handling References for anti-RYK Antibody (ABIN453358) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RYK Antibody Target Details RYK Application Details References for anti-RYK Antibody (ABIN453358) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RYK Antibody (ABIN453358)

Product Details anti-RYK Antibody Target Details RYK Application Details Handling Images back to top
Background publications

Trivier, Ganesan: "RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 23037-43, 2002

Katso, Russell, Ganesan: "Functional analysis of H-Ryk, an atypical member of the receptor tyrosine kinase family." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6427-40, 1999

Wang, Katso, Butler, Hanby, Poulsom, Jones, Sheer, Ganesan: "H-RYK, an unusual receptor kinase: isolation and analysis of expression in ovarian cancer." in: Molecular medicine (Cambridge, Mass.), Vol. 2, Issue 2, pp. 189-203, 1996

Images

Product Details anti-RYK Antibody Target Details RYK Application Details Handling References for anti-RYK Antibody (ABIN453358) back to top
Supplier Images
Western Blotting (WB) image for anti-RYK antibody (RYK Receptor-Like Tyrosine Kinase) (Middle Region) (ABIN453358) Western blot analysis of RYK antibody (Center C397) in Hela cell line lysates (35ug/l...