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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||85 Antibodies||15 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies||2 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Antigen||RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies|
|Epitope||AA 160-190 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RYK antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-RYK AntibodyTarget Details RYK Application Details Handling References for anti-RYK Antibody (ABIN392050) Images
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 AA from human RYK.|
Target Details RYKProduct Details anti-RYK Antibody Application Details Handling References for anti-RYK Antibody (ABIN392050) Images back to top
|Alternative Name||RYK (RYK Antibody Abstract)|
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Membrane, Single-pass type I membrane protein (By similarity). Nucleus (By similarity). Cytoplasm (By similarity). Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus (By similarity)
Observed in all the tissues examined.
Synonyms: Tyrosine-protein kinase RYK precursor
|Molecular Weight||67507 Da|
|Research Area||Stem Cells, Wnt, Tyrosine Kinases, Kinases/Phosphatases, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Pathways||RTK Signaling, WNT Signaling|
Application DetailsProduct Details anti-RYK Antibody Target Details RYK Handling References for anti-RYK Antibody (ABIN392050) Images back to top
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RYK Antibody Target Details RYK Application Details References for anti-RYK Antibody (ABIN392050) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.|
|Expiry Date||6 months|
References for anti-RYK Antibody (ABIN392050)Product Details anti-RYK Antibody Target Details RYK Application Details Handling Images back to top
|Product cited in:||
González, Fernández-Martos, Arenas et al.: "The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury." in: Journal of neurotrauma, Vol. 30, Issue 10, pp. 806-17, 2013 (PubMed).
Miyashita, Koda, Kitajo et al.: "Wnt-Ryk signaling mediates axon growth inhibition and limits functional recovery after spinal cord injury." in: Journal of neurotrauma, Vol. 26, Issue 7, pp. 955-64, 2009 (PubMed).
ImagesProduct Details anti-RYK Antibody Target Details RYK Application Details Handling References for anti-RYK Antibody (ABIN392050) back to top