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anti-Mouse (Murine) RYK antibody for Western Blotting

Recommended RYK Antibody (supplied by: Log in to see )

Antigen
RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
  • AW536699
  • D3S3195
  • d3s3195
  • ERK-3
  • JTK5
  • jtk5
  • JTK5A
  • jtk5a
  • MGC114645
  • RYK
  • ryk1
  • RYK1
  • Vik
  • wu:fb37b12
  • wu:fi05b06
  • zgc:158381
Epitope
AA 160-190
19
17
15
14
14
11
8
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
93
19
8
1
1
1
Host
Rabbit
66
23
10
3
Clonality
Polyclonal
Conjugate
This RYK antibody is un-conjugated
9
9
8
8
8
8
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
68
31
6
3
3
2
1
1
Supplier
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Catalog No. ABIN392050
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN264516 WB Mouse IgG1 Log in to see F35 P7 D7 F5 1
0.13398378 ABIN1538306 WB Rabbit Ig AA 321-348, Center Log in to see Polyclonal
0.13398378 ABIN2501805 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13398378 ABIN2501807 IHC WB Rabbit Ig Fraction Log in to see Polyclonal
0.13398378 ABIN307128 WB Mouse IgG1 Log in to see F35 P7 D7 F5 1
0.0011731827 ABIN359937 EIA IHC (p) WB Rabbit Ig AA 150-200 Log in to see Polyclonal 5
0.0011731827 ABIN1585167 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011731827 ABIN1807291 WB Rabbit AA 320-348 Log in to see Polyclonal
0.0011731827 ABIN325806 ELISA WB Sheep IgG Log in to see Polyclonal
0.0011731827 ABIN2627785 ELISA WB Biotin Rabbit IgG AA 287-533 Log in to see Polyclonal
0.0011731827 ABIN2627786 WB FITC Rabbit IgG AA 287-533 Log in to see Polyclonal
0.0011731827 ABIN1982809 ICC FACS WB Sheep IgG AA 35-211 Log in to see Polyclonal
0.0011731827 ABIN2355117 IC ELISA WB Sheep IgG AA 35-211 Log in to see Polyclonal
0.0011731827 ABIN3060809 ICC IF IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-RYK antibody for Western Blotting

Similar anti-RYK Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 85 Antibodies 15 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
Epitope AA 160-190
(19), (17), (15), (14), (14), (11), (8), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(93), (19), (8), (1), (1), (1)
Host Rabbit
(66), (23), (10), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This RYK antibody is un-conjugated
(9), (9), (8), (8), (8), (8)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (68), (31), (6), (3), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-RYK Antibody

Target Details RYK Application Details Handling References for anti-RYK Antibody (ABIN392050) Images
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 AA from human RYK.
Clone RB1491
Isotype Ig

Target Details RYK

Product Details anti-RYK Antibody Application Details Handling References for anti-RYK Antibody (ABIN392050) Images back to top
Antigen
Alternative Name RYK (RYK Antibody Abstract)
Background Function:
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Cellular Location:
Membrane, Single-pass type I membrane protein (By similarity). Nucleus (By similarity). Cytoplasm (By similarity). Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus (By similarity)

Tissue Location:
Observed in all the tissues examined.

Synonyms: Tyrosine-protein kinase RYK precursor
Molecular Weight 67507 Da
Gene ID 6259
UniProt P34925
Research Area Stem Cells, Wnt, Tyrosine Kinases, Kinases/Phosphatases, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-RYK Antibody Target Details RYK Handling References for anti-RYK Antibody (ABIN392050) Images back to top
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-RYK Antibody Target Details RYK Application Details References for anti-RYK Antibody (ABIN392050) Images back to top
Format Liquid
Concentration 1.57 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-RYK Antibody (ABIN392050)

Product Details anti-RYK Antibody Target Details RYK Application Details Handling Images back to top
Product cited in:

González, Fernández-Martos, Arenas, Rodríguez: "The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury." in: Journal of neurotrauma, Vol. 30, Issue 10, pp. 806-17, 2013

Miyashita, Koda, Kitajo, Yamazaki, Takahashi, Kikuchi, Yamashita: "Wnt-Ryk signaling mediates axon growth inhibition and limits functional recovery after spinal cord injury." in: Journal of neurotrauma, Vol. 26, Issue 7, pp. 955-64, 2009

Images

Product Details anti-RYK Antibody Target Details RYK Application Details Handling References for anti-RYK Antibody (ABIN392050) back to top
Supplier Images
Western Blotting (WB) image for anti-RYK antibody (RYK Receptor-Like Tyrosine Kinase) (AA 160-190) (ABIN392050) The anti-RYK Pab (ABIN392050) is used in Western blot to detect RYK in Jurkat cell ly...
Western Blotting (WB) image for anti-RYK antibody (RYK Receptor-Like Tyrosine Kinase) (AA 160-190) (ABIN392050) Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody (ABIN392050...
Immunohistochemistry (IHC) image for anti-RYK antibody (RYK Receptor-Like Tyrosine Kinase) (AA 160-190) (ABIN392050) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...