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anti-Dog (Canine) SHC1 antibody for Enzyme Immunoassay

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Epitope
pSer36
41
39
17
14
13
13
13
10
9
9
9
8
7
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
222
173
132
9
6
6
2
2
1
1
1
Host
Mouse
208
26
Clonality
Monoclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
172
83
62
56
49
38
8
8
7
3
3
1
1
Supplier
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Catalog No. ABIN126891
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN126888 EIA IHC (fro) WB Mouse IgG1 pTyr239, pTyr240 Log in to see 1-00E-003

Similar anti-SHC1 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine)
Western Blotting (WB) 165 Antibodies 116 Antibodies 10 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 7 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies 27 Antibodies
Immunohistochemistry (IHC) 86 Antibodies 50 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies 5 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies 56 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope pSer36
(41), (39), (17), (14), (13), (13), (13), (10), (9), (9), (9), (8), (7), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(222), (173), (132), (9), (6), (6), (2), (2), (1), (1), (1)
Host Mouse
(208), (26)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(172), (83), (62), (56), (49), (38), (8), (8), (7), (3), (3), (1), (1)
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling Images
Specificity This antibody specifically recognizes shc/p66 when it is phosphorylated at Serine 36. We recommend to Immunoprecipitate shc/p66 prior to detection with mab shc/p66-6E10.
Characteristics Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjugated to KLH
Clone 6-00E-010
Isotype IgG1

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SHC1 / SHC (SHC1 Antibody Abstract)
Background Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Molecular Weight 66 kDa
Gene ID 6464
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Application Notes Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: shc/p66 precipitated from EGF-treated HepG2 cells (See included Protocolbelow). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Cell Lysate: HepG2 shc/p66-IPFormulationshc/p66 immunoprecipitated with anti-shc from EGF stimulated HepG2 cells. Proteins wereeluted with SDS and lyophilized from PBS/SDS. ReconstitutionReconstitute by addition of 125 µl H2O. After complete solubilization add 125 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing.
Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (pSer36) (ABIN126891) anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pSer36) antibody