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anti-Human SHC1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • SHC1
  • SHC
  • SHCA
  • P66shc
  • si:dkey-246i14.4
  • wu:fc10h03
  • shc
  • p66shc
  • Shc
  • ShcA
  • p66
  • SHC (Src homology 2 domain containing) transforming protein 1
  • Shc1p
  • squalene--hopene cyclase
  • src homology 2 domain-containing transforming protein C1
  • SHC1
  • shc1
  • LOC100229782
  • Shc1
Epitope
AA 536-564, C-Term
41
39
19
17
15
15
14
10
9
8
7
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
224
177
139
10
6
5
2
2
1
1
1
1
1
Host
Rabbit
206
29
Clonality
Polyclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
162
78
66
58
44
35
11
7
5
4
4
3
1
1
Options
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Catalog No. ABIN3043923
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126889 IHC (fro) WB Mouse IgG1 pTyr317 Log in to see 1-50E-012
1 ABIN126887 IHC (fro) IF WB Mouse IgG1 C-Term Log in to see 11F6
1 ABIN126888 EIA IHC (fro) WB Mouse IgG1 pTyr239, pTyr240 Log in to see 1-00E-003
1 ABIN3044111 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 424-440, C-Term Log in to see Polyclonal
1 ABIN3032555 ICC IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-SHC1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (IF) 15 Antibodies 30 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies 66 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Western Blotting (WB) 75 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope AA 536-564, C-Term
(41), (39), (19), (17), (15), (15), (14), (10), (9), (8), (7), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(224), (177), (139), (10), (6), (5), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(206), (29)
Clonality
Polyclonal
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(162), (78), (66), (58), (44), (35), (11), (7), (5), (4), (4), (3), (1), (1)
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Sequence VDPEGVVRTK DHRFESVSHL ISYHMDNHL
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: SHC (Src homology 2 domain containing) transforming protein 1
Protein Name: SHC-transforming protein 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL), identical to the related mouse and rat sequences.
Isotype IgG

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SHC1 (SHC1 Antibody Abstract)
Background SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms: FLJ26504 antibody|MGC98812 antibody|p46 antibody|p52 antibody|p52 SHC antibody|p52SHC antibody|p66 antibody|p66 SHC antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC (Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain containing transforming protein C1 antibody|Src homology 2 domain-containing-transforming protein C1 antibody
Gene ID 6464
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat , The detection limit for SHC is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 536-564) (ABIN3043923) Anti- SHC1 Picoband antibody, IHC(P) IHC(P): Mouse Brain Tissue
Immunohistochemistry (IHC) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 536-564) (ABIN3043923) Anti- SHC1 Picoband antibody, IHC(F) IHC(F): Human Placenta Tissue
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 536-564) (ABIN3043923) anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 536-564), (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 536-564) (ABIN3043923) Anti- SHC1 Picoband antibody, IHC(P) IHC(P): Rat Brain Tissue
Immunohistochemistry (IHC) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 536-564) (ABIN3043923) Anti- SHC1 Picoband antibody, IHC(P) IHC(P): Human Lung Cancer Tissue