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anti-Mouse (Murine) SHC1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Reactivity
Human, Mouse (Murine), Rat (Rattus)
222
173
131
10
6
6
2
2
1
1
1
Host
Rabbit
207
27
Clonality
Polyclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
172
83
62
56
49
37
8
7
7
4
3
1
1
Supplier
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Catalog No. ABIN498175
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN197086 IF IHC (p) WB Rabbit pTyr427 Log in to see Polyclonal 3
0.00089690165 ABIN197590 IF IHC (p) WB Rabbit Tyr427 Log in to see Polyclonal 3
0.00089690165 ABIN271910 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.00089690165 ABIN302944 IHC (p) Rabbit IgG pTyr349 Log in to see Polyclonal
0.00089690165 ABIN3043923 IHC (fro) IHC (p) WB Rabbit IgG AA 536-564, C-Term Log in to see Polyclonal
0.00089690165 ABIN2854911 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.00089690165 ABIN745913 IF (p) IHC (p) WB Rabbit IgG AA 25-60, pSer36 Log in to see Polyclonal
0.00089690165 ABIN700990 IF (p) IHC (p) WB Rabbit IgG AA 480-520 Log in to see Polyclonal
0.00089690165 ABIN257557 ICC IF IHC (p) IHC WB Rabbit IgG Tyr427 Log in to see Polyclonal
0.00089690165 ABIN745973 IF (p) IHC (p) WB Rabbit IgG pTyr349, AA 330-370 Log in to see Polyclonal
0.00089690165 ABIN745915 IHC (p) WB Biotin Rabbit IgG AA 25-60, pSer36 Log in to see Polyclonal
0.00089690165 ABIN700992 IHC (p) WB Biotin Rabbit IgG AA 480-520 Log in to see Polyclonal
0.00089690165 ABIN700999 IHC (p) WB HRP Rabbit IgG AA 480-520 Log in to see Polyclonal
0.00089690165 ABIN745922 IHC (p) WB HRP Rabbit IgG AA 25-60, pSer36 Log in to see Polyclonal
0.00089690165 ABIN745930 IHC (p) WB Biotin Rabbit IgG pTyr349 Log in to see Polyclonal
0.00089690165 ABIN745945 IHC (p) WB Biotin Rabbit IgG pTyr427 Log in to see Polyclonal
0.00089690165 ABIN745937 IHC (p) WB HRP Rabbit IgG pTyr349 Log in to see Polyclonal
0.00089690165 ABIN745952 IHC (p) WB HRP Rabbit IgG pTyr427 Log in to see Polyclonal
0.00089690165 ABIN745960 IHC (p) WB Biotin Rabbit IgG pTyr317 Log in to see Polyclonal
0.00089690165 ABIN745967 IHC (p) WB HRP Rabbit IgG pTyr317 Log in to see Polyclonal

Similar anti-SHC1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 32 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 6 Antibodies
Immunofluorescence (IF) 18 Antibodies 35 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies 56 Antibodies
Immunohistochemistry (IHC) 46 Antibodies 50 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 79 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(222), (173), (131), (10), (6), (6), (2), (2), (1), (1), (1)
Host Rabbit
(207), (27)
Clonality
Polyclonal
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(172), (83), (62), (56), (49), (37), (8), (7), (7), (4), (3), (1), (1)
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling Images
Specificity This antibody detects endogenous levels of Shc protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SHC1 / SHC (SHC1 Antibody Abstract)
Background Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Molecular Weight approx. 46,52,66 kDa
Gene ID 6464
NCBI Accession NP_001123513
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoprecipitation: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (ABIN498175) Immunohistochemistry analyzes of Shc antibody in paraffin-embedded human brea...
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (ABIN498175) Western blot analysis of Shc antibody in extracts from HeLa cells.