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|Application / Reactivity||Human|
|Dot Blot (DB)||16 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||48 Antibodies|
|Functional Studies (Func)||3 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||46 Antibodies|
|Immunohistochemistry (IHC)||44 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||23 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies|
|Mass Cytometry (CyTOF)||1 Antibodies|
|Western Blotting (WB)||141 Antibodies|
|Antigen||TEK Tyrosine Kinase, Endothelial (TEK) Antibodies|
|Conjugate||This TEK antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TEK AntibodyTarget Details TEK Application Details Handling References for anti-TEK Antibody (ABIN359945) Images
|Specificity||This antiobdy recognizes TEK (TIE2).|
|Purification||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human TEK.|
Target Details TEKProduct Details anti-TEK Antibody Application Details Handling References for anti-TEK Antibody (ABIN359945) Images back to top
|Alternative Name||CD202b / TEK (TEK Antibody Abstract)|
|Background||The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK|
|Gene ID||7010, 9606|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK Antibody (ABIN359945) Images back to top
ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK Antibody (ABIN359945) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-TEK Antibody (ABIN359945)Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
DeBusk, Chen, Nishishita et al.: "Tie2 receptor tyrosine kinase, a major mediator of tumor necrosis factor alpha-induced angiogenesis in rheumatoid arthritis." in: Arthritis and rheumatism, Vol. 48, Issue 9, pp. 2461-71, 2003 (PubMed).
Poncet, Gasc, Janzer et al.: "Expression of Tie-2 in human peripheral and autonomic nervous system." in: Neuropathology and applied neurobiology, Vol. 29, Issue 4, pp. 361-9, 2003 (PubMed).
Sussman, Upalakalin, Roberts et al.: "Blood markers for vasculogenesis increase with tumor progression in patients with breast carcinoma." in: Cancer biology & therapy, Vol. 2, Issue 3, pp. 255-6, 2003 (PubMed).
Cascone, Audero, Giraudo et al.: "Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1." in: Blood, Vol. 102, Issue 7, pp. 2482-90, 2003 (PubMed).
Lee, Koh: "Shear stress activates Tie2 receptor tyrosine kinase in human endothelial cells." in: Biochemical and biophysical research communications, Vol. 304, Issue 2, pp. 399-404, 2003 (PubMed).
ImagesProduct Details anti-TEK Antibody Target Details TEK Application Details Handling References for anti-TEK Antibody (ABIN359945) back to top