anti-Human TEK antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended TEK Antibody (supplied by: Log in to see )

Antigen
TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK tyrosine kinase, endothelial
  • endothelial-specific receptor tyrosine kinase
  • TEK
  • LOC100356675
  • tek
  • Tek
Epitope
AA 450-500
30
28
25
24
23
23
19
17
14
14
14
11
10
7
6
6
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
378
179
84
7
6
3
1
Host
Rabbit
204
180
48
4
3
2
Clonality
Polyclonal
Conjugate
This TEK antibody is un-conjugated
29
22
19
18
15
13
10
10
9
7
7
7
7
7
6
6
6
5
5
5
5
4
4
4
4
4
4
4
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
269
195
152
136
63
52
43
33
22
13
8
7
6
5
4
4
4
2
1
Options
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Catalog No. ABIN674649
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1702572 IF (p) Cy3 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1706340 IF (p) Cy5.5 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1695310 IF (p) Alexa Fluor 488 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1695311 IF (p) Alexa Fluor 488 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1693491 IF (p) Alexa Fluor 350 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1693492 IF (p) Alexa Fluor 350 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1698946 IF (p) Alexa Fluor 647 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1697134 IF (p) Alexa Fluor 555 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1697135 IF (p) Alexa Fluor 555 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1698945 IF (p) Alexa Fluor 647 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1709962 IF (p) FITC Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1709963 IF (p) FITC Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1708151 IF (p) Cy7 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1708152 IF (p) Cy7 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1706341 IF (p) Cy5.5 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1704382 IF (p) Cy5 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN1704383 IF (p) Cy5 Rabbit IgG pTyr1108 Log in to see Polyclonal
1 ABIN1702571 IF (p) Cy3 Rabbit IgG pTyr1102 Log in to see Polyclonal
1 ABIN2814995 IF (p) Alexa Fluor 594 Rabbit IgG AA 450-500 Log in to see Polyclonal
1 ABIN2807033 IF (p) Alexa Fluor 594 Rabbit IgG pTyr1102 Log in to see Polyclonal

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
Epitope AA 450-500
(30), (28), (25), (24), (23), (23), (19), (17), (14), (14), (14), (11), (10), (7), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(378), (179), (84), (7), (6), (3), (1)
Host Rabbit
(204), (180), (48), (4), (3), (2)
Clonality
Polyclonal
Conjugate This TEK antibody is un-conjugated
(29), (22), (19), (18), (15), (13), (10), (10), (9), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(269), (195), (152), (136), (63), (52), (43), (33), (22), (13), (8), (7), (6), (5), (4), (4), (4), (2), (1)
Pubmed 4 references available
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Product Details anti-TEK Antibody

Target Details TEK Application Details Handling References for anti-TEK antibody (ABIN674649) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Tie2
Isotype IgG

Target Details TEK

Product Details anti-TEK Antibody Application Details Handling References for anti-TEK antibody (ABIN674649) Images back to top
Antigen
Alternative Name Tie2/Cd202b (TEK Antibody Abstract)
Background

Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.

Subcellular location: Extracellular

Synonyms: TIE2, VMCM, TIE-2, VMCM1, CD22B, Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p14 TEK, TEK

Gene ID 7010
UniProt Q02763
Pathways RTK Signaling, Growth Factor Binding

Application Details

Product Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK antibody (ABIN674649) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK antibody (ABIN674649) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-TEK antibody (ABIN674649)

Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
Product cited in:

Li, Liu, Liu, Wang, Zhang, Chen, Hui: "Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation." in: Neuroscience letters, Vol. 639, pp. 120-125, 2017 Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Xiang, Hu, Shen, Liu, Hua, Zan, Zu, Cui, Ye: "Bone marrow mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke in type 2 diabetic rats." in: Neuroscience letters, Vol. 644, pp. 62-66, 2017 Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Tekari, Chan, Sakai, Grad, Gantenbein: "Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells." in: Stem cell research & therapy, Vol. 7, Issue 1, pp. 75, 2016

Zhao, Hu, Xiang, Gu, Jin, Hua, Zhang, Liu, Zan, Zhang, Zu, Yang, Shi, Zhu, Xu, Cui, Ye: "Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke." in: Brain research, 2015

Images

Product Details anti-TEK Antibody Target Details TEK Application Details Handling References for anti-TEK antibody (ABIN674649) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (AA 450-500) antibody (ABIN674649) Formalin-fixed and paraffin embedded rat retina tissue labeled with Anti TIE2/CD202b ...