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anti-Human TEK antibody for Western Blotting

Recommended TEK Antibody (supplied by: Log in to see )

Antigen
TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK tyrosine kinase, endothelial
  • endothelial-specific receptor tyrosine kinase
  • TEK
  • LOC100356675
  • tek
  • Tek
Epitope
pTyr1108
29
25
22
22
19
19
17
14
14
13
13
10
7
6
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
334
147
70
6
6
2
1
Host
Rabbit
192
146
34
4
2
2
Clonality
Polyclonal
Conjugate
This TEK antibody is un-conjugated
25
19
16
14
12
11
8
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
229
179
131
112
52
45
42
34
20
11
9
7
4
4
4
3
2
1
Options
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Catalog No. ABIN1531402
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9499168 ABIN392059 IHC (p) WB Rabbit Ig AA 758-789, C-Term Log in to see Polyclonal 1
2.9499168 ABIN967565 IHC (fro) WB Mouse IgG1, kappa Extracellular Domain Log in to see 33 4
2.9499168 ABIN951222 WB Rabbit IgG AA 19-745 Log in to see Polyclonal 3
2.9499168 ABIN4900637 FACS WB Rabbit IgG pTyr992 Log in to see Polyclonal 4
2.9499168 ABIN2705294 WB Rabbit C-Term, pTyr1108 Log in to see Polyclonal
2.9499168 ABIN3043418 WB Rabbit IgG AA 641-830 Log in to see Polyclonal
2.9499168 ABIN3182332 ELISA WB Rabbit IgG pTyr992 Log in to see Polyclonal
2.9499168 ABIN393723 WB Mouse IgG2a kappa AA 701-800 Log in to see 3F8
2.9499168 ABIN393675 WB Mouse IgG2a kappa AA 701-800 Log in to see 3C1
2.9499168 ABIN1875052 WB Rabbit IgG Log in to see Polyclonal
2.9499168 ABIN449630 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see tek16
2.9499168 ABIN449626 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see tek2
2.9499168 ABIN3029103 IHC ELISA WB Rabbit Ig Fraction AA 758-789 Log in to see Polyclonal
2.9499168 ABIN1863205 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-116 Log in to see Polyclonal
2.9499168 ABIN4247753 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.9499168 ABIN1327048 ELISA WB Mouse IgG2a kappa AA 66-185, partial Log in to see 3A5
2.9499168 ABIN520827 ELISA WB Mouse IgG2a kappa AA 66-185 Log in to see 4G10
2.9499168 ABIN2504531 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
2.9499168 ABIN563140 ELISA WB Mouse IgG2a kappa AA 701-800, partial Log in to see 1A4
2.9499168 ABIN563142 ELISA WB Mouse IgG2a kappa AA 701-800, partial Log in to see 3C1

Top referenced anti-TEK antibody for Western Blotting

Similar anti-TEK Antibodies

Application / Reactivity Mouse (Murine) Human
Dot Blot (DB) 4 Antibodies 16 Antibodies
ELISA 30 Antibodies 169 Antibodies
ELISA (Detection) 4 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 9 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 70 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
Epitope pTyr1108
(29), (25), (22), (22), (19), (19), (17), (14), (14), (13), (13), (10), (7), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(334), (147), (70), (6), (6), (2), (1)
Host Rabbit
(192), (146), (34), (4), (2), (2)
Clonality
Polyclonal
Conjugate This TEK antibody is un-conjugated
(25), (19), (16), (14), (12), (11), (8), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(229), (179), (131), (112), (52), (45), (42), (34), (20), (11), (9), (7), (4), (4), (4), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-TEK Antibody

Target Details TEK Application Details Handling References for anti-TEK Antibody (ABIN1531402) Images
Specificity TIE2 (Phospho-Tyr1108) Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr1108.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1108.
Isotype IgG

Target Details TEK

Product Details anti-TEK Antibody Application Details Handling References for anti-TEK Antibody (ABIN1531402) Images back to top
Antigen
Alternative Name TIE2 (TEK Antibody Abstract)
Background Molecular weight of target protein: 125 kDa
Synonyms: Tyrosine-protein kinase receptor TIE-2, hTIE2, Tyrosine-protein kinase receptor TEK, p140 TEK, Tunica interna endothelial cell kinase, CD202b antigen
Molecular Weight 125 kDa
Gene ID 7010
OMIM 600195
UniProt Q02763
Research Area Organogenesis, Tyrosine Kinases
Pathways RTK Signaling

Application Details

Product Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK Antibody (ABIN1531402) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.89640 (NCBI Gene Symbol: TEK)

Restrictions For Research Use only

Handling

Product Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK Antibody (ABIN1531402) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-TEK Antibody (ABIN1531402)

Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
Background publications

Sjöblom, Jones, Wood, Parsons, Lin, Barber, Mandelker, Leary, Ptak, Silliman, Szabo, Buckhaults, Farrell, Meeh, Markowitz, Willis, Dawson, Willson, Gazdar, Hartigan, Wu, Liu, Parmigiani, Park et al.: "The consensus coding sequences of human breast and colorectal cancers. ..." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006

Ziegler, Bird, Schneringer, Schooley, Baum: "Molecular cloning and characterization of a novel receptor protein tyrosine kinase from human placenta." in: Oncogene, Vol. 8, Issue 3, pp. 663-70, 1993

Images

Product Details anti-TEK Antibody Target Details TEK Application Details Handling References for anti-TEK Antibody (ABIN1531402) back to top
Supplier Images
Western Blotting (WB) image for anti-TEK antibody (TEK Tyrosine Kinase, Endothelial) (pTyr1108) (ABIN1531402) Western blot analysis of extracts from NIH-3T3 cells, using TIE2 (Phospho-Tyr1108) An...
Immunofluorescence (IF) image for anti-TEK antibody (TEK Tyrosine Kinase, Endothelial) (pTyr1108) (ABIN1531402) Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. Th...