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Human TEK ELISA Kit

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Antigen
TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kits
  • AA517024
  • CD202B
  • Cd202b
  • DKFZp469E064
  • Hyk
  • TEK
  • Tie-2
  • TIE-2
  • tie-2
  • TIE2
  • tie2
  • Tie2
  • VMCM
  • VMCM1
Epitope
AA 23-745
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
28
18
8
3
3
2
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN411357
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN414924 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.94-60 ng/mL 0.94 ng/mL Log in to see
0.32666028 ABIN1380054 Sandwich ELISA Cell Lysate, Serum, Plasma 157-10000 pg/mL 157 pg/mL Log in to see
0.32666028 ABIN1030101 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.32666028 ABIN2708390 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.32666028 ABIN2065298 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012121415 ABIN510590 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012121415 ABIN456730 Serum, Plasma, Tissue Homogenate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012121415 ABIN852776 Serum, Plasma, Tissue Homogenate, Cell Lysate 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012121415 ABIN1380271 Cell ELISA Log in to see
0.0012121415 ABIN1381552 Cell ELISA Log in to see
0.0012121415 ABIN1112680 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 156-10000 pg/mL 156 pg/mL Log in to see
0.0012121415 ABIN625097 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012121415 ABIN2042679 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012121415 ABIN2115137 Cell ELISA Log in to see
0.0012121415 ABIN2065296 Cell Lysate, Serum, Plasma 157-10000 pg/mL 157 pg/mL Log in to see
0.0012121415 ABIN2685576 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.0012121415 ABIN2685577 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Log in to see
0.0012121415 ABIN2115136 Cell ELISA Log in to see
0.0012121415 ABIN2116362 6-400 μIU/mL 6 μIU/mL Log in to see
0.0012121415 ABIN1847059 Tissue Homogenate, Cell Lysate, Biological Fluids 156-10000 pg/mL 156 pg/mL Log in to see

Top referenced TEK ELISA Kit for ELISA

Similar TEK ELISA Kits

Application / Reactivity Human
ELISA 29 ELISA Kits

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kits
Epitope AA 23-745
Reactivity Human
Kits with alternative reactivity to:
(28), (18), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details TEK ELISA Kit

Target details Application Details Handling References for TEK Kit (ABIN411357) Images
Purpose For quantitative detection of human TIE2 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human TIE2
Cross-Reactivity (Details) There is cross-reactivates with human TIE1< 0.1 % .
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- human TIE2 antibod - 1
  • Lyophilized recombinant human TIE2 standar - 10ng/tubex2
  • Biotinylated anti- human TIE2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A23-K745

Target details

Product Details TEK ELISA Kit Application Details Handling References for TEK Kit (ABIN411357) Images back to top
Antigen
Alternative Name TEK (TEK ELISA Kit Abstract)
Background Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
Gene ID 7010
UniProt Q02763
Pathways RTK Signaling

Application Details

Product Details TEK ELISA Kit Target details Handling References for TEK Kit (ABIN411357) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human TIE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIE2 has been precoated onto 96-well plates. Standards(NSO, A23-K745) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIE2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIE2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 0.87, Standard deviation: 0.048, CV(%): 5.5
  • Sample 2: n=16, Mean(ng/ml): 2.75, Standard deviation: 0.132, CV(%): 4.8
  • Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.334, CV(%): 5.3,
  • Sample 1: n=24, Mean(ng/ml): 1.01, Standard deviation: 0.088, CV(%): 8.7
  • Sample 2: n=24, Mean(ng/ml): 2.88, Standard deviation: 0.19, CV(%): 6.6
  • Sample 3: n=24, Mean(ng/ml): 7.2, Standard deviation: 0.422, CV(%): 5.7
Restrictions For Research Use only

Handling

Product Details TEK ELISA Kit Target details Application Details References for TEK Kit (ABIN411357) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TEK Kit (ABIN411357)

Product Details TEK ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Sancakdar, Guven, Uysal, Deveci, Gültürk: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." in: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, 2015

Background publications

Zheng, Zhu, Bai, Wu, Jia, Hu: "Exercise improves recovery after ischemic brain injury by inducing the expression of angiopoietin-1 and Tie-2 in rats." in: The Tohoku journal of experimental medicine, Vol. 224, Issue 3, pp. 221-8, 2011

Arai, Hirao, Ohmura, Sato, Matsuoka, Takubo, Ito, Koh, Suda: "Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche." in: Cell, Vol. 118, Issue 2, pp. 149-61, 2004

Ramsden, Cocks, Shams, Nijjar, Watkinson, Sheppard, Ahmed, Eggo: "Tie-2 is expressed on thyroid follicular cells, is increased in goiter, and is regulated by thyrotropin through cyclic adenosine 3',5'-monophosphate." in: The Journal of clinical endocrinology and metabolism, Vol. 86, Issue 6, pp. 2709-16, 2001

Vikkula, Boon, Carraway, Calvert, Diamonti, Goumnerov, Pasyk, Marchuk, Warman, Cantley, Mulliken, Olsen: "Vascular dysmorphogenesis caused by an activating mutation in the receptor tyrosine kinase TIE2." in: Cell, Vol. 87, Issue 7, pp. 1181-90, 1997

Sato, Qin, Kozak, Audus: "Tie-1 and tie-2 define another class of putative receptor tyrosine kinase genes expressed in early embryonic vascular system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 20, pp. 9355-8, 1993

Images

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Supplier Images
ELISA image for TEK ELISA Kit (TEK Tyrosine Kinase, Endothelial) (ABIN411357) Human TIE2 PicoKine ELISA Kit standard curve