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anti-Mouse (Murine) TIE1 antibody for Enzyme Immunoassay

Recommended TIE1 Antibody (supplied by: Log in to see )

Antigen
tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies
  • D430008P04Rik
  • JTK14
  • TIE
  • tie-1
Epitope
N-Term
19
17
14
13
12
11
6
5
4
4
4
3
3
3
2
2
1
Reactivity
Human, Mouse (Murine)
132
40
28
2
Host
Rabbit
97
36
2
1
Clonality
Polyclonal
Conjugate
This TIE1 antibody is un-conjugated
7
5
5
5
4
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
94
75
28
21
13
11
6
5
4
3
2
Supplier
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Catalog No. ABIN359947
$ 390.50
Plus shipping costs $45.00

Top referenced anti-TIE1 antibody for Enzyme Immunoassay

Similar anti-TIE1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 10 Antibodies 77 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 6 Antibodies
Flow Cytometry (FACS) 3 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 23 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies

General

Antigen tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies
Epitope N-Term
(19), (17), (14), (13), (12), (11), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1)
Reactivity Human, Mouse (Murine)
(132), (40), (28), (2)
Host Rabbit
(97), (36), (2), (1)
Clonality
Polyclonal
Conjugate This TIE1 antibody is un-conjugated
(7), (5), (5), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(94), (75), (28), (21), (13), (11), (6), (5), (4), (3), (2)
Pubmed 4 references available
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Product Details anti-TIE1 Antibody

Target Details TIE1 Application Details Handling References for anti-TIE1 Antibody (ABIN359947) Images
Specificity This antibody detects TIE1 at N-term.
Purification Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human TIE.
Isotype Ig

Target Details TIE1

Product Details anti-TIE1 Antibody Application Details Handling References for anti-TIE1 Antibody (ABIN359947) Images back to top
Antigen
Alternative Name TIE1 (TIE1 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1
Molecular Weight 125089 Da
Gene ID 7075, 9606
UniProt P35590
Research Area Lineage Markers, Tyrosine Kinases
Pathways RTK Signaling

Application Details

Product Details anti-TIE1 Antibody Target Details TIE1 Handling References for anti-TIE1 Antibody (ABIN359947) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details References for anti-TIE1 Antibody (ABIN359947) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-TIE1 Antibody (ABIN359947)

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling Images back to top
Background publications

Verstovsek, Kantarjian, Manshouri, OBrien, Faderl, Talpaz, Cortes, Albitar: "Prognostic significance of Tie-1 protein expression in patients with early chronic phase chronic myeloid leukemia." in: Cancer, Vol. 94, Issue 5, pp. 1517-21, 2002

Tsiamis, Morris, Marron, Brindle: "Vascular endothelial growth factor modulates the Tie-2:Tie-1 receptor complex." in: Microvascular research, Vol. 63, Issue 2, pp. 149-58, 2002

Sato, Tozawa, Deutsch, Wolburg-Buchholz, Fujiwara, Gendron-Maguire, Gridley, Wolburg, Risau, Qin: "Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation." in: Nature, Vol. 376, Issue 6535, pp. 70-4, 1995

Partanen, Armstrong, Mäkelä, Korhonen, Sandberg, Renkonen, Knuutila, Huebner, Alitalo: "A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains." in: Molecular and cellular biology, Vol. 12, Issue 4, pp. 1698-707, 1992

Images

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling References for anti-TIE1 Antibody (ABIN359947) back to top
Supplier Images
 image for anti-TIE1 antibody (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1) (N-Term) (ABIN359947) anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (N-Term) antibody
 image for anti-TIE1 antibody (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1) (N-Term) (ABIN359947) anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (N-Term) antibody (Image 2)