Rat (Rattus) TRKA ELISA Kit

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Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
  • trk
  • trka
  • TRKA
  • MTC
  • TRK
  • TRK1
  • Trk-A
  • p140-TrkA
  • C80751
  • Tkr
  • TrkA
  • Trk
  • CTRKA
  • trkA
  • CAPM1
  • CFTD
  • NEM1
  • OK/SW-cl.5
  • TM-5
  • TM3
  • TM30
  • TM30nm
  • TM5
  • TPMsk3
  • hscp30
  • neurotrophic tyrosine kinase, receptor, type 1
  • tropomyosin 3
  • ntrk1
  • NTRK1
  • LOC100354292
  • Ntrk1
  • TPM3
Epitope
AA 33-418
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
19
18
12
1
Method Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN921109
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.374001 ABIN2708683 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
12.374001 ABIN2114672 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate Log in to see
1 ABIN857900 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.625-40 ng/mL 0.625 ng/mL Log in to see
1 ABIN1380759 Cell ELISA Log in to see
1 ABIN1380760 Cell ELISA Log in to see
1 ABIN1381567 Cell ELISA Log in to see
1 ABIN1112693 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2042711 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
1 ABIN2685116 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2114632 Cell ELISA Log in to see
1 ABIN2114633 Cell ELISA Log in to see
1 ABIN1847070 Serum, Plasma, Biological Fluids 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2114072 Cell ELISA Log in to see
1 ABIN2685115 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2116383 Sandwich ELISA 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN1136650 Log in to see
1 ABIN2948298 Log in to see
1 ABIN1720665 Sandwich ELISA Log in to see

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
Epitope AA 33-418
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(19), (18), (12), (1)
Method Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details TRKA ELISA Kit

Target details Application Details Handling ProductDetails: References for TRKA Kit (ABIN921109) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat trkA
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: A33-P418
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A33-P418

Target details

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Antigen
Alternative Name NTRK1 (NTRK1 ELISA Kit Abstract)
Background

Protein Function: Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. .

Background: Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

Synonyms: High affinity nerve growth factor receptor,2.7.10.1,Neurotrophic tyrosine kinase receptor type 1,Slow nerve growth factor receptor,p140-TrkA,Trk-A,Ntrk1,Trk, Trka,

Full Gene Name: High affinity nerve growth factor receptor

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Early endosome membrane, Single-pass type I membrane protein. Late endosome membrane, Single-pass type I membrane protein. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes.
Gene ID 59109
UniProt P35739
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Tissue Specificity: Isoform Trka-II is primarily expressed in neuronal cells, isoform Trka-I is found in non-neuronal tissues.

Plate Pre-coated
Protocol rat TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards(NSO, A33-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TrkA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat TrkA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TrkA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 595, Standard deviation: 32.13, CV(%): 5.4
  • Sample 2: n=16, Mean(pg/ml): 3622, Standard deviation: 166.6, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 6653, Standard deviation: 452.4, CV(%): 6.8,
  • Sample 1: n=24, Mean(pg/ml): 614, Standard deviation: 38.68, CV(%): 6.3
  • Sample 2: n=24, Mean(pg/ml): 3125, Standard deviation: 162.5, CV(%): 5.2
  • Sample 3: n=24, Mean(pg/ml): 7234, Standard deviation: 542.6, CV(%): 7.5
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for TRKA Kit (ABIN921109)

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Background publications

Georgieva, de Pablo, Sanchis, Comella, Llovera: "Ubiquitination of TrkA by Nedd4-2 regulates receptor lysosomal targeting and mediates receptor signaling." in: Journal of neurochemistry, Vol. 117, Issue 3, pp. 479-93, 2011

Benito-Gutiérrez, Garcia-Fernàndez, Comella: "Origin and evolution of the Trk family of neurotrophic receptors." in: Molecular and cellular neurosciences, Vol. 31, Issue 2, pp. 179-92, 2006

Images

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Supplier Images
ELISA image for Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kit (ABIN921109) Rat TrkA PicoKine ELISA Kit standard curve