anti-Human TSC1 antibody for ELISA

Recommended TSC1 Antibody (supplied by: Log in to see )

Antigen
Tuberous Sclerosis 1 (TSC1) Antibodies
  • tsc1
  • wu:fc38b04
  • LAM
  • TSC
  • hamartin
  • mKIAA0243
  • tuberous sclerosis 1
  • tuberous sclerosis 1b
  • TSC1
  • tsc1b
  • tsc1
  • Tsc1
Epitope
C-Term
49
43
40
40
40
40
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
16
12
12
10
9
7
7
7
7
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
463
380
173
28
7
6
5
1
1
Host
Goat
744
57
18
2
Clonality
Polyclonal
Conjugate
This TSC1 antibody is un-conjugated
67
67
67
64
64
64
5
5
5
5
5
3
3
3
3
3
3
2
2
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
532
518
166
65
39
34
29
21
17
8
4
2
2
1
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Catalog No. ABIN185619
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN251283 IHC (p) IHC ELISA WB Goat C-Term, Isoform 1 Log in to see Polyclonal 2
1 ABIN574381 IHC (p) ELISA WB Goat AA 1150-1163 Log in to see Polyclonal
1 ABIN961783 IHC (p) IP ELISA WB Mouse IgG1 Log in to see
1 ABIN1585745 ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2466406 IHC ELISA WB Goat C-Term Log in to see Polyclonal
1 ABIN1740408 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal
1 ABIN2278842 ELISA WB Alkaline Phosphatase (AP) Goat IgG C-Term Log in to see Polyclonal
1 ABIN2278843 ELISA WB APC Goat IgG C-Term Log in to see Polyclonal
1 ABIN2278844 ELISA WB Biotin Goat IgG C-Term Log in to see Polyclonal
1 ABIN2278845 ELISA WB FITC Goat IgG C-Term Log in to see Polyclonal
1 ABIN2278847 ELISA WB PE Goat IgG C-Term Log in to see Polyclonal
1 ABIN2278839 IHC ELISA WB Goat C-Term Log in to see Polyclonal
1 ABIN2278841 ELISA WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN5627135 ELISA ICC IF WB Rabbit IgG AA 220-270, all Isoforms Log in to see Polyclonal
1 ABIN203563 ELISA Rabbit AA 656-672 Log in to see Polyclonal
1 ABIN203562 ELISA Rabbit AA 99-115 Log in to see Polyclonal
1 ABIN3029281 DB ELISA Rabbit Ig Fraction pSer1080 Log in to see Polyclonal
1 ABIN2278854 DB ELISA Rabbit IgG pSer505 Log in to see Polyclonal
1 ABIN2278861 IHC ELISA Rabbit IgG Ser505 Log in to see Polyclonal
1 ABIN1031146 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal

General

Antigen Tuberous Sclerosis 1 (TSC1) Antibodies
Epitope C-Term
(49), (43), (40), (40), (40), (40), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (16), (12), (12), (10), (9), (7), (7), (7), (7), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(463), (380), (173), (28), (7), (6), (5), (1), (1)
Host Goat
(744), (57), (18), (2)
Clonality
Polyclonal
Conjugate This TSC1 antibody is un-conjugated
(67), (67), (67), (64), (64), (64), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(532), (518), (166), (65), (39), (34), (29), (21), (17), (8), (4), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-TSC1 Antibody

Target Details TSC1 Application Details Handling ProductDetails: References for anti-TSC1 antibody (ABIN185619) Images
Blocking Peptide Blocking peptide for this product available: ABIN369665
Specificity This antibody is expected to recognise isoform 1 (NP_000359.1) only.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-QLHIMDYNETHHEH, from the C Terminus of the protein sequence according to NP_000359.1.

Target Details TSC1

Product Details anti-TSC1 Antibody Application Details Handling ProductDetails: References for anti-TSC1 antibody (ABIN185619) Images back to top
Antigen
Alternative Name Hamartin / TSC1 (TSC1 Antibody Abstract)
Background Synonyms: TSC1, hamartin, tuberous sclerosis 1, HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor
Gene ID 7248, 64930
NCBI Accession NP_000359
Pathways RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-TSC1 Antibody Target Details TSC1 Handling ProductDetails: References for anti-TSC1 antibody (ABIN185619) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 130 kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22,9(12):1721-7, PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details ProductDetails: References for anti-TSC1 antibody (ABIN185619) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

ProductDetails: References for anti-TSC1 antibody (ABIN185619)

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details Handling Images back to top
Background publications

Ballif, Roux, Gerber, MacKeigan, Blenis, Gygi: "Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 3, pp. 667-72, 2005

Chan, Zhang, Roberts, Jozwiak, Wieslawa, Lewin-Kowalik, Kotulska, Kwiatkowski: "Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation." in: Journal of neuropathology and experimental neurology, Vol. 63, Issue 12, pp. 1236-42, 2004

Goncharova, Goncharov, Noonan, Krymskaya: "TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase." in: The Journal of cell biology, Vol. 167, Issue 6, pp. 1171-82, 2004

Images

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details Handling ProductDetails: References for anti-TSC1 antibody (ABIN185619) back to top
Supplier Images
 image for anti-Tuberous Sclerosis 1 (TSC1) (C-Term) antibody (ABIN185619) ABIN185619 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). P...