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anti-Rat (Rattus) TSC1 antibody for Enzyme Immunoassay

Recommended TSC1 Antibody (supplied by: Log in to see )

Antigen
Tuberous Sclerosis 1 (TSC1) Antibodies
  • hamartin
  • LAM
  • mKIAA0243
  • TSC
  • tsc1
  • wu:fc38b04
Epitope
Middle Region, both Isoforms
51
40
40
40
40
27
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
17
15
12
12
12
12
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
4
3
3
3
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
424
358
152
5
1
1
1
1
1
1
Host
Rabbit
727
37
18
1
Clonality
Polyclonal
Conjugate
This TSC1 antibody is un-conjugated
67
67
67
64
64
64
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
532
489
152
62
33
32
17
14
7
5
3
2
1
Supplier
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Catalog No. ABIN501071
$ 401.50
Plus shipping costs $45.00

Top referenced anti-TSC1 antibody for Enzyme Immunoassay

Similar anti-TSC1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 76 Antibodies 247 Antibodies
ELISA 69 Antibodies 203 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 7 Antibodies
Immunofluorescence (IF) 6 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Western Blotting (WB) 40 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen Tuberous Sclerosis 1 (TSC1) Antibodies
Epitope Middle Region, both Isoforms
(51), (40), (40), (40), (40), (27), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (17), (15), (12), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(424), (358), (152), (5), (1), (1), (1), (1), (1), (1)
Host Rabbit
(727), (37), (18), (1)
Clonality
Polyclonal
Conjugate This TSC1 antibody is un-conjugated
(67), (67), (67), (64), (64), (64), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(532), (489), (152), (62), (33), (32), (17), (14), (7), (5), (3), (2), (1)
Pubmed 4 references available
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Product Details anti-TSC1 Antibody

Target Details TSC1 Application Details Handling References for anti-TSC1 Antibody (ABIN501071) Images
Specificity This antibody detects TSC1 / Hamartin at Center.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.
Isotype IgG

Target Details TSC1

Product Details anti-TSC1 Antibody Application Details Handling References for anti-TSC1 Antibody (ABIN501071) Images back to top
Antigen
Alternative Name TSC1 / Hamartin (TSC1 Antibody Abstract)
Background Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.Synonyms: KIAA0243, TSC, Tuberous sclerosis 1 protein
Gene ID 7248
Pathways RTK Signaling, AMPK Signaling

Application Details

Product Details anti-TSC1 Antibody Target Details TSC1 Handling References for anti-TSC1 Antibody (ABIN501071) Images back to top
Application Notes ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details References for anti-TSC1 Antibody (ABIN501071) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-TSC1 Antibody (ABIN501071)

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details Handling Images back to top
Background publications

Inoki, Ouyang, Li, Guan: "Signaling by target of rapamycin proteins in cell growth control." in: Microbiology and molecular biology reviews : MMBR, Vol. 69, Issue 1, pp. 79-100, 2005

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003

Tabancay, Gau, Machado, Uhlmann, Gutmann, Guo, Tamanoi: "Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39921-30, 2003

Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003

Images

Product Details anti-TSC1 Antibody Target Details TSC1 Application Details Handling References for anti-TSC1 Antibody (ABIN501071) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TSC1 antibody (Tuberous Sclerosis 1) (Middle Region) (ABIN501071) Immunocytochemistry of TSC1 in EL4 cells with this product at 2 μg/ml
Western Blotting (WB) image for anti-TSC1 antibody (Tuberous Sclerosis 1) (Middle Region) (ABIN501071) Western blot analysis of TSC1 in EL4 cell lysate with this product at 1 μg/ml in the ...