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anti-Human TSC2 antibody for Immunofluorescence

Recommended TSC2 Antibody (supplied by: Log in to see )

Antigen
Tuberous Sclerosis 2 (TSC2) Antibodies
  • fc16b02
  • LAM
  • Nafld
  • Rc
  • Tcs2
  • TSC2
  • TSC4
  • tuberin
  • wu:fc16b02
Epitope
N-Term
41
40
34
27
27
27
24
23
22
22
20
20
20
20
20
20
20
20
20
19
18
17
16
15
15
15
13
13
13
13
11
8
8
6
6
6
6
6
6
6
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
523
377
209
6
Host
Rabbit
697
24
Clonality
Polyclonal
Conjugate
This TSC2 antibody is un-conjugated
51
51
51
43
42
42
10
10
9
9
9
9
9
9
9
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
387
291
218
148
101
75
62
24
7
6
4
2
1
Supplier
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Catalog No. ABIN501075
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1881949 DB IF Rabbit pSer1387 Log in to see Polyclonal 6
0.32040194 ABIN1532423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.32040194 ABIN2705307 IC IF IHC WB Rabbit C-Term, pSer1462 Log in to see Polyclonal
0.32040194 ABIN256563 ICC IF IHC (p) IHC Rabbit IgG pSer664 Log in to see Poly6359
0.32040194 ABIN2500763 IF IHC ELISA WB Rabbit IgG Ser939 Log in to see Polyclonal
0.0010840179 ABIN389800 DB IF Rabbit Ig pSer939 Log in to see Polyclonal
0.0010840179 ABIN389817 DB IF Rabbit Ig pSer664 Log in to see Polyclonal
0.0010840179 ABIN389864 DB IF Rabbit Ig pSer1420 Log in to see Polyclonal
0.0010840179 ABIN389863 DB IF Rabbit Ig pSer1798 Log in to see Polyclonal
0.0010840179 ABIN390324 IF IHC (p) WB Rabbit Ig AA 1776-1805, Ser1798 Log in to see Polyclonal
0.0010840179 ABIN218353 IF IHC (p) Rabbit IgG pSer664 Log in to see Polyclonal
0.0010840179 ABIN2888710 IF IHC (p) ELISA WB Rabbit IgG AA 905-954 Log in to see Polyclonal
0.0010840179 ABIN303312 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0010840179 ABIN2469754 IF IHC Rabbit IgG pSer664 Log in to see Polyclonal
0.0010840179 ABIN1740414 IF IHC (p) Rabbit IgG pSer664 Log in to see Polyclonal
0.0010840179 ABIN3187372 ELISA IF IHC WB Rabbit IgG Tyr228 Log in to see Polyclonal
0.0010840179 ABIN2891840 IF IHC WB Rabbit Phe933 Log in to see Polyclonal
0.0010840179 ABIN3182844 ELISA IF IHC Rabbit IgG pThr1462 Log in to see Polyclonal
0.0010840179 ABIN2576146 IF IHC (p) WB Rabbit Ser1798 Log in to see Polyclonal
0.0010840179 ABIN408095 ICC IF IHC (p) IHC WB Rabbit IgG Phe933 Log in to see Polyclonal

Top referenced anti-TSC2 antibody for Immunofluorescence

Similar anti-TSC2 Antibodies

Application / Reactivity Mouse (Murine) Human
Dot Blot (DB) 152 Antibodies 120 Antibodies
ELISA 167 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 7 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 101 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Western Blotting (WB) 101 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 2 Antibodies

General

Antigen Tuberous Sclerosis 2 (TSC2) Antibodies
Epitope N-Term
(41), (40), (34), (27), (27), (27), (24), (23), (22), (22), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (18), (17), (16), (15), (15), (15), (13), (13), (13), (13), (11), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(523), (377), (209), (6)
Host Rabbit
(697), (24)
Clonality
Polyclonal
Conjugate This TSC2 antibody is un-conjugated
(51), (51), (51), (43), (42), (42), (10), (10), (9), (9), (9), (9), (9), (9), (9), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(387), (291), (218), (148), (101), (75), (62), (24), (7), (6), (4), (2), (1)
Pubmed 4 references available
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Product Details anti-TSC2 Antibody

Target Details TSC2 Application Details Handling References for anti-TSC2 Antibody (ABIN501075) Images
Specificity This antibody detects TSC2 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Immunogen TSC2 (NT) was raised against a 14 amino acid peptide from near the amino terminus of human TSC2.
Isotype IgG

Target Details TSC2

Product Details anti-TSC2 Antibody Application Details Handling References for anti-TSC2 Antibody (ABIN501075) Images back to top
Antigen
Alternative Name Tuberin / TSC2 (TSC2 Antibody Abstract)
Background Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). At least three isoforms of TSC2 exist.Synonyms: TSC4, Tuberous sclerosis 2 protein
Gene ID 7249
NCBI Accession NP_000539
Pathways RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-TSC2 Antibody Target Details TSC2 Handling References for anti-TSC2 Antibody (ABIN501075) Images back to top
Application Notes ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TSC2 Antibody Target Details TSC2 Application Details References for anti-TSC2 Antibody (ABIN501075) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-TSC2 Antibody (ABIN501075)

Product Details anti-TSC2 Antibody Target Details TSC2 Application Details Handling Images back to top
Background publications

Inoki, Ouyang, Li, Guan: "Signaling by target of rapamycin proteins in cell growth control." in: Microbiology and molecular biology reviews : MMBR, Vol. 69, Issue 1, pp. 79-100, 2005

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003

Tabancay, Gau, Machado, Uhlmann, Gutmann, Guo, Tamanoi: "Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39921-30, 2003

Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003

Images

Product Details anti-TSC2 Antibody Target Details TSC2 Application Details Handling References for anti-TSC2 Antibody (ABIN501075) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TSC2 antibody (Tuberous Sclerosis 2) (N-Term) (ABIN501075) Immunocytochemistry of TSC2 in L1210 cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-TSC2 antibody (Tuberous Sclerosis 2) (N-Term) (ABIN501075) Western blot analysis of TSC2 in L1210 cell lysate with this product at (A) 2 and (B)...