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anti-Human TYRO3 antibody for Immunofluorescence

Recommended TYRO3 Antibody (supplied by: Log in to see )

Antigen
TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
  • AI323366
  • brt
  • Brt
  • BYK
  • byk
  • DKFZp469I0432
  • Dtk
  • dtk
  • Etk-2
  • ksy
  • LOC100231598
  • MGC145734
  • REK
  • Rse
  • RSE
  • rse
  • Sky
  • sky
  • tif
  • Tif
  • TYRO3
  • xksy
  • Xksy
Epitope
AA 100-250, Internal Region
17
13
12
11
8
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
170
59
24
4
1
Host
Rabbit
98
72
12
Clonality
Polyclonal
Conjugate
This TYRO3 antibody is un-conjugated
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
127
72
42
41
22
18
17
15
12
10
6
2
2
Supplier
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Catalog No. ABIN440474
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.27038345 ABIN2452233 FACS IF IP Mouse IgG2a Log in to see 5B7
0.27038345 ABIN1109418 IF WB Mouse IgG1 Log in to see 1C10E8 3
0.27038345 ABIN1109420 EIA IF Mouse IgG1 Log in to see 6D6F10 2
0.27038345 ABIN2500874 IF ELISA Mouse IgG1 AA 138-321 Log in to see 6D6F10
0.27038345 ABIN931484 IF ELISA Mouse IgG1 AA 138-321 Log in to see 6D6F10
0.27038345 ABIN2498212 IF IHC Mouse Log in to see 64CT15-6-2-3-1
0.27038345 ABIN931451 IF ELISA WB Mouse IgG1 Log in to see 1C10E8-9A3F2
0.27038345 ABIN2500875 IF ELISA WB Mouse IgG1 Log in to see 1C10E8-9A3F2
0.27038345 ABIN4306338 ICC IF IHC IHC (p) IP WB Mouse IgG2b Log in to see 2C4
0.27038345 ABIN4306337 ELISA ICC IF WB Mouse IgG1 Log in to see 1C10E8
0.27038345 ABIN4306336 ELISA ICC IF Mouse IgG1 Log in to see 6D6F10
0.0011012722 ABIN467120 IF ELISA Mouse IgG1 Log in to see 6D6F10
0.0011012722 ABIN1501603 FACS IF IP Mouse IgG2a Log in to see 5B7
0.0011012722 ABIN1501605 IF IHC (p) IP WB Mouse IgG2b Log in to see 2C4
0.0011012722 ABIN1501604 IF IHC (p) IP WB Mouse IgG1 Log in to see 2D11
0.0011012722 ABIN2452511 IF IP IHC WB Mouse IgG2b Log in to see 2C4
0.0011012722 ABIN2452433 IF IP IHC WB Mouse IgG1 Log in to see 2D11
0.0011012722 ABIN2452434 FACS IF IP IHC WB Mouse IgG1 Log in to see 9B12
0.0011012722 ABIN387820 IF IHC (p) Mouse Log in to see 64CT15-6-2-3-1
0.0011012722 ABIN1501602 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see 9B12

Top referenced anti-TYRO3 antibody for Immunofluorescence

Similar anti-TYRO3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 71 Antibodies
ELISA (Capture) 1 Antibodies 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 6 Antibodies
Flow Cytometry (FACS) 12 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 16 Antibodies
Immunofluorescence (IF) 7 Antibodies 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 39 Antibodies

General

Antigen TYRO3 Protein Tyrosine Kinase (TYRO3) Antibodies
Epitope AA 100-250, Internal Region
(17), (13), (12), (11), (8), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(170), (59), (24), (4), (1)
Host Rabbit
(98), (72), (12)
Clonality
Polyclonal
Conjugate This TYRO3 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(127), (72), (42), (41), (22), (18), (17), (15), (12), (10), (6), (2), (2)
Pubmed 1 reference available
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Product Details anti-TYRO3 Antibody

Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]

Target Details TYRO3

Product Details anti-TYRO3 Antibody Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Antigen
Alternative Name Dtk/TYRO3 (TYRO3 Antibody Abstract)
Background Gene Symbol: TYRO3
Molecular Weight Theoretical MW: 96 kDa
Gene ID 7301
UniProt Q06418
Pathways RTK Signaling

Application Details

Product Details anti-TYRO3 Antibody Target Details TYRO3 Handling References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Application Notes Western Blot 2 μg/mL, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:200
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for TYRO3 Antibody Procedure Guide for NBP1-28635 - Tyro3 AntibodyProcedure Guide for NBP1-28635- Tyro3 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-Tyro3 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - Tyro3 Antibody - Page 1
Restrictions For Research Use only

Handling

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details References for anti-TYRO3 Antibody (ABIN440474) Images back to top
Format Liquid
Concentration 1.19 mg/mL
Buffer PBS, 30 % glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TYRO3 Antibody (ABIN440474)

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling Images back to top
Product cited in:

Chan, Carrera Silva, De Kouchkovsky, Joannas, Hao, Hu, Huntsman, Eng, Licona-Limón, Weinstein, Herbert, Craft, Flavell, Repetto, Correale, Burchard, Torgerson, Ghosh, Rothlin: "The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity." in: Science (New York, N.Y.), Vol. 352, Issue 6281, pp. 99-103, 2016 Further details: Flow Cytometry

Images

Product Details anti-TYRO3 Antibody Target Details TYRO3 Application Details Handling References for anti-TYRO3 Antibody (ABIN440474) back to top
Supplier Images
Western Blotting (WB) image for anti-TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250) (ABIN440474) anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody
Immunofluorescence (IF) image for anti-TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250) (ABIN440474) Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of...