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|Application / Reactivity||Pig (Porcine)||Human|
|ELISA||17 Antibodies||134 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||22 Antibodies|
|Blocking Reagent (BR)||1 Antibodies|
|Enzyme Immunoassay (EIA)||20 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Reactivity||Cow (Bovine), Human, Pig (Porcine) Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN451484) Images
|Cross-Reactivity (Details)||Mixed results in Cow,Pig (IHC only). Customers have reported success staining sheep tissue (see review).|
|Purification||Protein G purified|
|Immunogen||Recombinant human VEGF189 expressed in E.coli|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling References for anti-VEGF Antibody (ABIN451484) Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
Vascular endothelial growth factor (VEGF) was recently identified as a secreted,direct-acting mitogen specific for vascular endothelial cells and capable of stimulatingangiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparentlyarising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 arepotent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1induces apoptosis, VEGF protects endothelial cells from apoptosis. We found thatTGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGFsynthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogatesTGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates anunexpected role of VEGF and indicates that VEGF can be pharmacologically convertedinto an apoptotic factor, a novel approach to antiangiogenesis therapy (2). Alternate Names: anti-Vascular Endothelial Growth Factor antibody, anti-Vascular Permeability Factorantibody, anti-VEGFA antibody, anti-VPF antibody.
Gene Symbol: VEGFA
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF Antibody (ABIN451484) Images back to top
This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Radioimmunoassay was reported in scientific literature. In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.
Recommended dilutions: ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:10-1:500, Radioimmunoassay, Western Blot 0.5-2µg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF Antibody (ABIN451484) Images back to top
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||4 °C short term. Aliquot and store at -20 °C long term.|
References for anti-VEGF Antibody (ABIN451484)Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
|Product cited in:||
Mannheim, Versari, Daghini et al.: "Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularization." in: American journal of physiology. Heart and circulatory physiology, Vol. 292, Issue 5, pp. H2449-58, 2007 (PubMed).
Ferrari, Pintucci, Seghezzi et al.: "VEGF, a prosurvival factor, acts in concert with TGF-beta1 to induce endothelial cell apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 46, pp. 17260-5, 2006 (PubMed).
Zhang, Scott, Turley et al.: "Validation of anti-vascular endothelial growth factor (anti-VEGF) antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium." in: The Journal of pathology, Vol. 185, Issue 4, pp. 402-8, 1998 (PubMed).
Houck, Ferrara, Winer et al.: "The vascular endothelial growth factor family: identification of a fourth molecular species and characterization of alternative splicing of RNA." in: Molecular endocrinology (Baltimore, Md.), Vol. 5, Issue 12, pp. 1806-14, 1992 (PubMed).