anti-Human VEGF antibody for Functional Studies

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Epitope
Isoform 165
24
19
19
15
15
12
10
10
10
9
7
6
6
6
6
6
5
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
304
116
110
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Mouse
272
98
24
4
2
1
1
Clonality
Monoclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
296
197
81
58
26
26
25
18
18
17
10
10
7
5
4
2
2
2
1
1
Supplier
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Catalog No. ABIN155173
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181548 EIA Func WB Goat Log in to see Polyclonal 1
1 ABIN115672 EIA Func IP Rabbit AA 27-191, Isoform 165 Log in to see Polyclonal 3
1 ABIN115673 EIA Func IP Rabbit AA 27-191, Isoform 165 Log in to see Polyclonal 3
1 ABIN181154 EIA Func WB Mouse IgG1 Log in to see
1 ABIN116251 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN116252 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN296523 Func ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN2745578 Func ELISA WB Mouse IgG1 Log in to see 3(6D3)

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope Isoform 165
(24), (19), (19), (15), (15), (12), (10), (10), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(304), (116), (110), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(272), (98), (24), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(296), (197), (81), (58), (26), (26), (25), (18), (18), (17), (10), (10), (7), (5), (4), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN155173) Images
Specificity The antibody recognizes VEGF-A.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein G Chromatography
Immunogen Recombinant Human VEGF165 protein (45 kDa)
Clone 3
Isotype IgG1

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN155173) Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 7422
NCBI Accession NP_001020537
UniProt P15692
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN155173) Images back to top
Application Notes ELISA: 1-15 μg/mL. Western blot: 2-10 μg/mL. Neutralization: Blocks the VEGF-A induced proliferation of ACE cell at a 1: 6 Molar ratio ofligand to antibody. Inhibits the binding of VEGF-A to the VEGF receptors 1 (Flt-1) and 2 (KDR).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN155173) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer H2O, Trehalose, Na-Phosphate, Polysorbart20
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody, thought stable at RT, is best stored desiccated below 0 °C. Reconstituted anti-VEGF-A is stable at 2-8 °C for one month or (in aliquots) at - 20 °C for longer.

References for anti-VEGF antibody (ABIN155173)

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Background publications

Kim, Li, Winer, Armanini, Gillett, Phillips, Ferrara: "Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo." in: Nature, Vol. 362, Issue 6423, pp. 841-4, 1993

Kim, Li, Houck, Winer, Ferrara: "The vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies." in: Growth factors (Chur, Switzerland), Vol. 7, Issue 1, pp. 53-64, 1992