anti-Human VEGF antibody for Immunoprecipitation

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Epitope
AA 27-191, Isoform 165
24
19
19
15
15
12
10
10
10
9
7
6
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
304
116
110
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Rabbit
271
99
24
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunoprecipitation (IP)
297
197
81
58
26
26
25
18
17
17
10
10
7
5
4
2
2
2
1
1
Supplier
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Catalog No. ABIN115672
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115673 EIA Func IP Rabbit AA 27-191, Isoform 165 Log in to see Polyclonal 3
1 ABIN1078646 ICC IHC IP WB Rabbit IgG AA 28-191 Log in to see Polyclonal
1 ABIN2682748 IP WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1495081 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN1495082 IP Sepharose Rabbit N-Term Log in to see Polyclonal
1 ABIN5590719 BP ELISA FACS IP WB Mouse IgG1 kappa Log in to see A-4-6-1
1 ABIN1502611 IF IP WB Mouse kappa, IgG1 Log in to see JH121
1 ABIN5552620 IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal
1 ABIN385720 IF IP WB Mouse IgG1, kappa Log in to see JH121
1 ABIN226622 IP ELISA WB Mouse IgM Log in to see
1 ABIN1589726 IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN645823 IP ELISA WB Mouse IgM Log in to see

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope AA 27-191, Isoform 165
(24), (19), (19), (15), (15), (12), (10), (10), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(304), (116), (110), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(271), (99), (24), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunoprecipitation (IP)
(297), (197), (81), (58), (26), (26), (25), (18), (17), (17), (10), (10), (7), (5), (4), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN115672) Images
Specificity The antibody will detect Human VEGF-A.
Purification Protein A Affinity Chromatography
Immunogen Highly purified Recombinant Human VEGF165 [Ala27-Arg191] produced in Insect cells (Cat.-No DA3514X)

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN115672) Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 7422
NCBI Accession NP_001020537
UniProt P15692
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN115672) Images back to top
Application Notes Neutralization: 1-2.5 μg/mL, to yield one-half maximal inhibition (ND50) of the biologicalactivity of human VEGF (50 ng/mL). ELISA: 0.5 μg/mL, allows the detection of 0.5-1.0 ng/well of recombinant human VEGF165 orother VEGF splice forms. Immunoprecipitation: 1-5 μg/mL lysate or reaction volume.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN115672) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.4 containing no preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

References for anti-VEGF antibody (ABIN115672)

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Background publications

Kremer, Breier, Risau, Plate: "Up-regulation of flk-1/vascular endothelial growth factor receptor 2 by its ligand in a cerebral slice culture system." in: Cancer research, Vol. 57, Issue 17, pp. 3852-9, 1997

Fiebich, Jäger, Schöllmann, Weindel, Wilting, Kochs, Marmé, Hug, Weich: "Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells." in: European journal of biochemistry / FEBS, Vol. 211, Issue 1-2, pp. 19-26, 1993

Breier, Albrecht, Sterrer, Risau: "Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation." in: Development (Cambridge, England), Vol. 114, Issue 2, pp. 521-32, 1992