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anti-Human VEGF antibody for Western Blotting

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • eVEGF120
  • eVEGF164
  • MVCD1
  • vefg
  • VEGF
  • Vegf
  • vegf
  • VEGF-A
  • vegf-a
  • Vegf120
  • Vegf164
  • VEGF164
  • Vegf188
  • vegfa
  • Vpf
  • VPF
  • vpf
  • wu:fj82c06
Epitope
AA 147-176, Middle Region
27
24
17
13
13
10
10
9
9
7
6
6
6
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
263
99
94
25
19
12
11
6
2
1
1
Host
Rabbit
269
71
22
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
45
26
15
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
263
201
66
59
23
22
15
14
13
13
10
6
3
2
2
1
Supplier
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Catalog No. ABIN955508
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN656118 WB Rabbit Ig AA 140-168, Center Log in to see Polyclonal 1
0.12303223 ABIN521322 PLA ELISA WB Mouse IgG2a kappa AA 27-130, partial Log in to see 3F7 1
0.12303223 ABIN1109472 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.12303223 ABIN2788641 IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN3042324 IHC (p) WB Rabbit IgG AA 27-191 Log in to see Polyclonal
0.12303223 ABIN3044494 IHC (p) WB Rabbit IgG AA 33-48, N-Term Log in to see Polyclonal
0.12303223 ABIN258536 ICC FACS IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see EP1176Y
0.12303223 ABIN1876455 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1585685 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN1995399 IHC (p) ELISA WB Rabbit IgG AA 1-209 Log in to see Polyclonal
0.12303223 ABIN802829 IHC ELISA WB Rabbit Log in to see Polyclonal 1
0.12303223 ABIN2506673 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2493814 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.12303223 ABIN3187662 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN3201330 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 28-191 Log in to see
0.12303223 ABIN1078646 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 208-327 Log in to see Polyclonal
0.12303223 ABIN521321 WB Mouse AA 1-147, full length Log in to see Polyclonal
0.12303223 ABIN926640 WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2424297 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2406506 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-VEGF antibody for Western Blotting

Similar anti-VEGF Antibodies

Application / Reactivity Human
ELISA 132 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Functional Studies (Func) 11 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunoprecipitation (IP) 11 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope AA 147-176, Middle Region
(27), (24), (17), (13), (13), (10), (10), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(263), (99), (94), (25), (19), (12), (11), (6), (2), (1), (1)
Host Rabbit
(269), (71), (22), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(45), (26), (15), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(263), (201), (66), (59), (23), (22), (15), (14), (13), (13), (10), (6), (3), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN955508) Images
Specificity This antibody detects VEGF-A (Center).
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein A column followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 147-176 amino acids from the Central region of human VEGFA
Isotype Ig

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling References for anti-VEGF Antibody (ABIN955508) Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 7422
NCBI Accession NP_001020537
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF Antibody (ABIN955508) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF Antibody (ABIN955508) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.

References for anti-VEGF Antibody (ABIN955508)

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Background publications

Shrivastava-Ranjan, Rollin, Spiropoulou: "Andes virus disrupts the endothelial cell barrier by induction of vascular endothelial growth factor and downregulation of VE-cadherin." in: Journal of virology, Vol. 84, Issue 21, pp. 11227-34, 2010

Kim, Kim, Park, Park, Kim, Kim, Park, Song, Kang: "VEGF polymorphisms in early cervical cancer susceptibility, angiogenesis, and survival." in: Gynecologic oncology, Vol. 119, Issue 2, pp. 232-6, 2010

Yang, Andresen, Yang, Zhang, Li, Li, Wang: "Association of vascular endothelial growth factor -634C/G polymorphism and diabetic retinopathy in type 2 diabetic Han Chinese." in: Experimental biology and medicine (Maywood, N.J.), Vol. 235, Issue 10, pp. 1204-11, 2010

Huez, Bornes, Bresson, Créancier, Prats: "New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation." in: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 12, pp. 2197-210, 2001

Tee, Jaffe: "A precursor form of vascular endothelial growth factor arises by initiation from an upstream in-frame CUG codon." in: The Biochemical journal, Vol. 359, Issue Pt 1, pp. 219-26, 2001

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN955508) back to top
Supplier Images
Western Blotting (WB) image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (AA 147-176) (ABIN955508) VEGFA Antibody (Center) western blot analysis in K562 cell line lysates (35 µg/lane)....