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anti-Mouse (Murine) VEGF antibody for Enzyme Immunoassay

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • eVEGF120
  • eVEGF164
  • MVCD1
  • vefg
  • VEGF
  • Vegf
  • vegf
  • VEGF-A
  • vegf-a
  • Vegf120
  • Vegf164
  • VEGF164
  • Vegf188
  • vegfa
  • Vpf
  • VPF
  • vpf
  • wu:fj82c06
Reactivity
Human, Mouse (Murine)
263
99
93
25
19
12
11
6
2
1
1
Host
Rabbit
269
71
22
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
45
26
15
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
263
201
66
58
23
22
15
14
13
13
10
6
3
2
2
1
Supplier
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Catalog No. ABIN155190
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN155189 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN155188 EIA WB Rabbit Log in to see Polyclonal 3
0.0008870071 ABIN116331 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008870071 ABIN116332 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008870071 ABIN115675 EIA WB Rabbit N-Term Log in to see Polyclonal
0.0008870071 ABIN115674 EIA WB Rabbit N-Term Log in to see Polyclonal
0.0008870071 ABIN116334 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN116333 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-VEGF antibody for Enzyme Immunoassay

Similar anti-VEGF Antibodies

Application / Reactivity Mouse (Murine)
ELISA 30 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 57 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Human, Mouse (Murine)
(263), (99), (93), (25), (19), (12), (11), (6), (2), (1), (1)
Host Rabbit
(269), (71), (22), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(45), (26), (15), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(263), (201), (66), (58), (23), (22), (15), (14), (13), (13), (10), (6), (3), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN155190) Images
Specificity The antibody recognizes Human and Murine VEGF-A under reducing and non-reducing conditions.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Protein A Chromatography.
Immunogen Recombinant Murine VEGF164 Dimer (Cat.-No DA3518)

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling References for anti-VEGF Antibody (ABIN155190) Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternate transcriptional splice variants, encoding either freely secreted or cell associated isoforms, have been characterized. There is also evidence for the use of non AUG (CUG) translation initiation sites upstream of, and in frame with the first AUG, leading to additional isoforms.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 22339
NCBI Accession NP_001020421
UniProt Q00731
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF Antibody (ABIN155190) Images back to top
Application Notes ELISA: To detect Murine VEGF-A by direct ELISA a concentration of at least 0.5 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 0.5- 1.0 ng/well of recombinant murine VEGF164 or other VEGF-A splice forms. Western blot: To detect murine VEGF-A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01 % Proteinase K, 15 min atRT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF Antibody (ABIN155190) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives and stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-VEGF Antibody (ABIN155190)

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Background publications

Kremer, Breier, Risau, Plate: "Up-regulation of flk-1/vascular endothelial growth factor receptor 2 by its ligand in a cerebral slice culture system." in: Cancer research, Vol. 57, Issue 17, pp. 3852-9, 1997

Fiebich, Jäger, Schöllmann, Weindel, Wilting, Kochs, Marmé, Hug, Weich: "Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells." in: European journal of biochemistry / FEBS, Vol. 211, Issue 1-2, pp. 19-26, 1993

Breier, Albrecht, Sterrer, Risau: "Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation." in: Development (Cambridge, England), Vol. 114, Issue 2, pp. 521-32, 1992