anti-Mouse (Murine) VEGF antibody for Functional Studies

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Mouse (Murine)
305
116
109
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Rabbit
271
99
24
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
296
197
81
57
26
26
25
18
18
17
10
10
7
5
4
2
2
2
1
1
Supplier
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Catalog No. ABIN116334
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116333 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Mouse (Murine)
(305), (116), (109), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(271), (99), (24), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(296), (197), (81), (57), (26), (26), (25), (18), (18), (17), (10), (10), (7), (5), (4), (2), (2), (2), (1), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Specificity Reacts with Vascular Endothelial Growth Factor (VEGF).
Purification Affinity Chromatography.
Immunogen E.coli derived highly pure (> 98%) recombinant Murine VEGF

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGF-A (VEGFA Antibody Abstract)
Background VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Gene ID 22339
NCBI Accession NP_001020421
UniProt Q00731
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect mVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith biotinylated Anti-Murine VEGF (PP1113B) as a detection antibody, allows the detectionof at least 0.2-0.4 ng/well of recombinant mVEGF. Western BlotTo detect mVEGF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or at -20 °C for longer.