anti-Mouse (Murine) VEGF antibody for Neutralization

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Mouse (Murine)
281
114
108
29
24
14
13
10
3
3
3
2
1
1
1
1
1
1
Host
Rabbit
263
82
23
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
6
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
282
190
76
51
26
25
17
17
17
15
13
7
7
5
3
2
2
1
Supplier
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Catalog No. ABIN465273
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465294 ELISA IHC Neut WB Biotin Rabbit Log in to see Polyclonal

Similar anti-VEGF Antibodies

Application / Reactivity Mouse (Murine)
ELISA 32 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 67 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Mouse (Murine)
(281), (114), (108), (29), (24), (14), (13), (10), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(263), (82), (23), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
(282), (190), (76), (51), (26), (25), (17), (17), (17), (15), (13), (7), (7), (5), (3), (2), (2), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Specificity Mouse VEGF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Murine VEGF.

Type of Immunogen: Cell extract

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGFA / VEGF (VEGFA Antibody Abstract)
Background Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1
Gene ID 7422
UniProt P15692
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.08 - 0.12 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5 μg/mL for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mVEGF (10.00 ng/mL), a concentration of 0.08-0.12 μg/mL of this antibody is required. ELISA: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine VEGF (LS-C104524) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 2)
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 3)
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 4)
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 5)
 image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (ABIN465273) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 6)