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|Application / Reactivity||Human||Rabbit|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||207 Antibodies||8 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||14 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||45 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Epitope||Isoform 121 Alternatives|
|Reactivity||Human, Rabbit Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Functional Studies (Func)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN180646) Images
This antinbody detects Vascular Endothelial Growth Factor (VEGF).
Cellular Localization: Cytoplasmic, cell surface, and extracellular matrix.
|Characteristics||Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor|
|Purification||Protein G Chromatography.|
|Immunogen||Recombinant human VEGF121. Remarks: Mol. Wt. of Antigen: 19-22 kDa (reduced)|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling References for anti-VEGF Antibody (ABIN180646) Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
|Background||VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF Antibody (ABIN180646) Images back to top
Immunohistology (Formalin/Paraffin): 1/50 for 30 min. Staining of formalin-fixed,paraffin-embedded tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 8.0 for10-20 min followed by cooling at RT for 20 min. Positive Control: Angiosarcoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF Antibody (ABIN180646) Images back to top
|Buffer||10 mM PBS, pH 7.4, 0.09 % Sodium Azide, 0.2 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-VEGF Antibody (ABIN180646)Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Brown, Berse, Jackman, Tognazzi, Guidi, Dvorak, Senger, Connolly, Schnitt: "Expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in breast cancer." in: Human pathology, Vol. 26, Issue 1, pp. 86-91, 1995
ImagesProduct Details anti-VEGF Antibody Target Details VEGF Application Details Handling References for anti-VEGF Antibody (ABIN180646) back to top