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anti-Rabbit VEGF antibody for Immunohistochemistry (Frozen Sections)

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • eVEGF120
  • eVEGF164
  • MVCD1
  • vefg
  • VEGF
  • Vegf
  • vegf
  • VEGF-A
  • vegf-a
  • Vegf120
  • Vegf164
  • VEGF164
  • Vegf188
  • vegfa
  • Vpf
  • VPF
  • vpf
  • wu:fj82c06
Epitope
AA 1-120
27
24
17
13
13
11
10
9
9
7
6
6
6
5
5
5
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rabbit
264
99
94
25
19
12
11
5
2
1
1
Host
Guinea Pig
270
71
22
4
1
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
45
26
15
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
263
200
66
58
24
22
14
13
13
13
10
6
3
2
2
1
Supplier
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Catalog No. ABIN1863281
$ 376.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN2120270 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-VEGF Antibodies

Application / Reactivity Rabbit
ELISA 3 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Epitope AA 1-120
(27), (24), (17), (13), (13), (11), (10), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(264), (99), (94), (25), (19), (12), (11), (5), (2), (1), (1)
Host Guinea Pig
(270), (71), (22), (4), (1), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(45), (26), (15), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(263), (200), (66), (58), (24), (22), (14), (13), (13), (13), (10), (6), (3), (2), (2), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLLYLHHAKW SQAAPMAEEG DNKPHEVVKF MEVYRRSYCQ PIETLVDIFQ EYPDEIEYIF KPSCVPLVRC GGCCNDESLE CVPTEEFNVT MQIMRIKPHQ GQHIGEMSFL QHNKCECRPK
Specificity It has been selected for its ability to recognize VEGFA in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant VEGFA expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against VEGFA.
Isotype IgG

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vascular Endothelial Growth Factor A (VEGFA Antibody Abstract)
Pathways RTK Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant VEGFA (Leu1~Lys120) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-VEGF antibody (Vascular Endothelial Growth Factor A) (AA 1-120) (ABIN1863281) anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 1-120) antibody