anti-Rat (Rattus) VEGF antibody for Neutralization

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Rat (Rattus)
305
115
110
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Rabbit
271
99
24
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
296
196
81
58
26
26
26
18
17
17
11
10
7
5
4
2
2
2
1
1
Supplier
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Catalog No. ABIN465335
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465348 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Rat (Rattus)
(305), (115), (110), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(271), (99), (24), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA, Western Blotting (WB)
(296), (196), (81), (58), (26), (26), (26), (18), (17), (17), (11), (10), (7), (5), (4), (2), (2), (2), (1), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Specificity Rat VEGF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Rat VEGF.

Type of Immunogen: Cell extract

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGFA / VEGF (VEGFA Antibody Abstract)
Background Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1
Gene ID 7422
UniProt P15692
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat VEGF (LS-C104553) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
 image for anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (ABIN465335) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody
 image for anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (ABIN465335) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 2)
 image for anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (ABIN465335) anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (Image 3)