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Mouse (Murine) VEGF ELISA Kit

Recommended VEGF Product (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) ELISA Kits
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
19
18
15
10
9
7
6
6
5
3
2
2
2
Methode Type
Sandwich ELISA
Detection Range
2-1000 pg/mL
Minimum Detection Limit
2 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625186
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.426762 ABIN424307 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.8-500 pg/mL 7.8 pg/mL Log in to see 10
1 ABIN365363 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 3.906-250 pg/mL 3.906 pg/mL Log in to see 5
1 ABIN3032851 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-1000 pg/mL 4.1 pg/mL Log in to see
1 ABIN491836 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN456761 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN1573048 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4948123 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 15.6-1.000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1059774 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see
1 ABIN2685871 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN4948124 Sandwich ELISA Log in to see
1 ABIN4987897 Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum 3.906-250 pg/mL 3.906 pg/mL Log in to see
1 ABIN4987896 Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum 3.906-250 pg/mL 3.906 pg/mL Log in to see
1 ABIN4987153 Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum 3.906-250 pg/mL 3.906 pg/mL Log in to see
1 ABIN4973726 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2540304 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
1 ABIN2869902 Log in to see
1 ABIN2952374 7.8-500 pg/mL 7.8 pg/mL Log in to see

Top referenced VEGF ELISA Kit for ELISA

  • Mouse (Murine) VEGF ELISA Kit for Sandwich ELISA - ABIN625186 (28 Publications): Yamamoto, Fujita, Tanaka, Kojima, Kanatani, Ishihara, Tachibana: Low oxygen tension enhances proliferation and maintains stemness of adipose tissue-derived stromal cells. in BioResearch open access 2013 (PubMed)

  • Mouse (Murine) VEGF ELISA Kit for Sandwich ELISA - ABIN424307 (10 Publications): Obrador, Benlloch, Pellicer, Asensi, Estrela: Intertissue flow of glutathione (GSH) as a tumor growth-promoting mechanism: interleukin 6 induces GSH release from hepatocytes in metastatic B16 melanoma-bearing mice. in The Journal of biological chemistry 2011 (PubMed)

  • Mouse (Murine) VEGF ELISA Kit for Sandwich ELISA - ABIN365363 (5 Publications): Cheng, Hu, Lv, Ling, Jiang: Erythropoietin improves the efficiency of endothelial progenitor cell therapy after myocardial infarction in mice: effects on transplanted cell survival and autologous endothelial progenitor cell mobilization. in The Journal of surgical research 2012 (PubMed)

Similar VEGF ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 18 ELISA Kits

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(19), (18), (15), (10), (9), (7), (6), (6), (5), (3), (2), (2), (2)
Methode Type Sandwich ELISA
Detection Range 2-1000 pg/mL
Minimum Detection Limit 2 pg/mL
Application ELISA
Pubmed 20 references available
Supplier Log in to see

Product Details VEGF ELISA Kit

Target details Application Details Handling References for VEGF Kit (ABIN625186) Images
Purpose Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo).
Sensitivity < 2 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details VEGF ELISA Kit Application Details Handling References for VEGF Kit (ABIN625186) Images back to top
Antigen
Alternative Name VEGF-A (VEGFA ELISA Kit Abstract)
Background Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Gene ID 22339
UniProt Q00731
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling

Application Details

Product Details VEGF ELISA Kit Target details Handling References for VEGF Kit (ABIN625186) Images back to top
Application Notes Recommended Dilution for serum and plasma samples3 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 3 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 25 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 40 µL VEGF standard from the vial of Item C, into a tube with 960 µL Assay Diluent A or 1x Assay Diluent B to prepare a 1,000 pg/mL stock standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200 µL 200 µL 200 µL 200 µL 40 µL standard + 960.0 µL 200myl 1,000 400 160 64 25.6 10.2 4.1 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) before use. HRP-Streptavidin concentrate should be diluted 160-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 8 ml 1x Assay Diluent B to prepare a 160-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse VEGF concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse VEGF concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.1 1 10
Sensitivity: The minimum detectable dose of VEGF is typically less than 2 pg/mL.
Recovery: Recovery was determined by spiking various levels of mouse VEGF into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 87.67 79-102 Plasma 89.32 81-103 Cell culture media 94.29 83-105
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 96 97 95 Range ( %) 83-103 84-103 82-102 1:4 Average % of Expected 94 98 97 Range ( %) 82-102 84-104 83-103
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details VEGF ELISA Kit Target details Application Details References for VEGF Kit (ABIN625186) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for VEGF Kit (ABIN625186)

Product Details VEGF ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Huey, Smith, Sulaeman, Breen: "Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 120, Issue 2, pp. 188-95, 2016

Li, Wang, Huang, Zhang, Yao, Wang, Lv, An, Corrigan, Huang, Ying: "Distinct sustained structural and functional effects of interleukin-33 and interleukin-25 on the airways in a murine asthma surrogate." in: Immunology, Vol. 145, Issue 4, pp. 508-18, 2015

Pala, Atilgan, Ozkan, Kavak, Ilhan, Akpolat, Sapmaz: "Effect of varying doses of tamoxifen on ovarian histopathology, serum VEGF, and endothelin 1 levels in ovarian hyperstimulation syndrome: an experimental study." in: Drug design, development and therapy, Vol. 9, pp. 1761-6, 2015

Patel, Thaker: "The effects of A2B receptor modulators on vascular endothelial growth factor and nitric oxide axis in chronic cyclosporine nephropathy." in: Journal of pharmacology & pharmacotherapeutics, Vol. 6, Issue 3, pp. 147-53, 2015

Martin, Nguyen, Molinolo, Gutkind: "Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene." in: Oncogene, Vol. 33, Issue 18, pp. 2405-12, 2014

Wei, Yu, Lee, Chen, Taylor, Deveau, Yu, Wei: "Regulatory role of the JNK-STAT1/3 signaling in neuronal differentiation of cultured mouse embryonic stem cells." in: Cellular and molecular neurobiology, Vol. 34, Issue 6, pp. 881-93, 2014

Abbas, Borman, Terzi, Terzi, Bayraktar, Ozkan, Yazc: "Inhibition of the Notch Pathway Promotes Flap Survival by Inducing Functional Neoangiogenesis." in: Annals of plastic surgery, 2014

Cai, Zhou, Zhou, Lou: "Hypoxic conditioned medium from rat cerebral cortical cells enhances the proliferation and differentiation of neural stem cells mainly through PI3-K/Akt pathways." in: PLoS ONE, Vol. 9, Issue 11, pp. e111938, 2014

Yamamoto, Fujita, Tanaka, Kojima, Kanatani, Ishihara, Tachibana: "Low oxygen tension enhances proliferation and maintains stemness of adipose tissue-derived stromal cells." in: BioResearch open access, Vol. 2, Issue 3, pp. 199-205, 2013

Hartwich, Orr, Ng, Spence, Morton, Davidoff: "HIF-1? activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts." in: Journal of pediatric surgery, Vol. 48, Issue 1, pp. 39-46, 2013

Said, Frierson, Sanchez-Carbayo, Brekken, Theodorescu: "Loss of SPARC in bladder cancer enhances carcinogenesis and progression." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 751-66, 2013

Saldaña, Crespo, Bensiamar, Arruebo, Vilaboa: "Mechanical forces regulate stem cell response to surface topography." in: Journal of biomedical materials research. Part A, 2013

Xiao, Guo, Chen, El-Gohary, Wiersch, Gaffar, Prasadan, Shiota, Gittes: "Hypoglycemia reduces vascular endothelial growth factor A production by pancreatic beta cells as a regulator of beta cell mass." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8636-46, 2013

Brunner, Huber, Weinberger, Vallaster, Wollenweber, Gerbitz, Hacker, Franz: "Migration of bone marrow-derived cells and improved perfusion after treatment with erythropoietin in a murine model of myocardial infarction." in: Journal of cellular and molecular medicine, Vol. 16, Issue 1, pp. 152-9, 2012

Juffer, Jaspers, Lips, Bakker, Klein-Nulend: "Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes." in: American journal of physiology. Endocrinology and metabolism, Vol. 302, Issue 4, pp. E389-95, 2012

George, Rajoria, Suriano, Mittleman, Tiwari: "Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence." in: Molecular cancer, Vol. 11, pp. 80, 2012

Altman, Nguyen, Patel, Fambrough, Beedle, Hardman, Murph: "Regulator of G-Protein Signaling 5 Reduces HeyA8 Ovarian Cancer Cell Proliferation and Extends Survival in a Murine Tumor Model." in: Biochemistry research international, Vol. 2012, pp. 518437, 2012

Jones, Antonelli, Masko, Broadwater, Lascola, Fels, Dewhirst, Dyck, Nagendran, Flores, Betof, Nelson, Pollak, Dash, Young, Freedland: "Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 113, Issue 2, pp. 263-72, 2012

Sawai, Murata, Koto, Matsui, Horie, Ashihara, Maekawa, Fushiki, Kubo: "Effects of low-intensity pulsed ultrasound on osteosarcoma and cancer cells." in: Oncology reports, Vol. 28, Issue 2, pp. 481-6, 2012

Clarke, Al Ahmad, Lee, Parham, Auckland, Fertala, Kahle, Shaw, Roberts, Bix: "Perlecan Domain V induces VEGf secretion in brain endothelial cells through integrin α5β1 and ERK-dependent signaling pathways." in: PLoS ONE, Vol. 7, Issue 9, pp. e45257, 2012

Images

Product Details VEGF ELISA Kit Target details Application Details Handling References for VEGF Kit (ABIN625186) back to top
Supplier Images
ELISA image for VEGF ELISA Kit (Vascular Endothelial Growth Factor A) (ABIN625186) Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit