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Human VEGFC ELISA Kit

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Antigen
Vascular Endothelial Growth Factor C (VEGFC) ELISA Kits
  • AW228853
  • Flt4-L
  • vegf-c
  • VEGF-C
  • VEGFC
  • VRP
  • xvegf-c
Epitope
AA 103-227
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
25
9
9
3
3
3
2
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411382
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.306289 ABIN414935 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 2
0.306289 ABIN1117760 Sandwich ELISA Serum, Plasma, Biological Fluids 109.375-7000 pg/mL 109.38 pg/mL Log in to see
0.306289 ABIN2708410 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.306289 ABIN2115152 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012155632 ABIN365328 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
0.0012155632 ABIN3033002 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 13.7-10000 pg/mL 13.7 pg/mL Log in to see
0.0012155632 ABIN511650 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012155632 ABIN456766 Sandwich ELISA Body Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155632 ABIN625369 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 9
0.0012155632 ABIN1030135 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155632 ABIN1112703 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155632 ABIN2685878 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155632 ABIN2685877 Sandwich ELISA Plasma (citrate), Cell Culture Supernatant, Serum 0.23-15 ng/mL 0.23 ng/mL Log in to see
0.0012155632 ABIN2685879 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155632 ABIN2042722 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155632 ABIN1847081 Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155632 ABIN2540310 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012155632 ABIN3014850 Sandwich ELISA Log in to see
0.0012155632 ABIN2110828 Sandwich ELISA Log in to see
0.0012155632 ABIN2952376 109.375-7000 pg/mL 109.375 pg/mL Log in to see

Top referenced VEGFC ELISA Kit for ELISA

Similar VEGFC ELISA Kits

Application / Reactivity Human
ELISA 24 ELISA Kits

General

Antigen Vascular Endothelial Growth Factor C (VEGFC) ELISA Kits
Epitope AA 103-227
Reactivity Human
Kits with alternative reactivity to:
(25), (9), (9), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 11 references available
Supplier Log in to see

Product Details VEGFC ELISA Kit

Target details Application Details Handling References for VEGFC Kit (ABIN411382) Images
Purpose For quantitative detection of human VEGF-C in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <3pg/mL
Components
  • 96-well plate precoated with anti- human VEGF-C antibod - 1
  • Lyophilized recombinant human VEGF-C standar - 10ng/tubex2
  • Biotinylated anti- human VEGF-C antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: T103-R227

Target details

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Antigen
Alternative Name VEGFC (VEGFC ELISA Kit Abstract)
Background Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.
Gene ID 7424
UniProt P49767
Research Area Cancer, Angiogenesis, Growth Factors
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human VEGF-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF-C has been precoated onto 96-well plates. Standards(NSO, T103-R227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGF-C amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL,2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGF-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human VEGF-C standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 198, Standard deviation: 6.93, CV(%): 3.5
  • Sample 2: n=16, Mean(pg/ml): 1915, Standard deviation: 80.43, CV(%): 4.2
  • Sample 3: n=16, Mean(pg/ml): 3096, Standard deviation: 145.5, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 236, Standard deviation: 10.62, CV(%): 4.5
  • Sample 2: n=24, Mean(pg/ml): 2534, Standard deviation: 134.3, CV(%): 5.3
  • Sample 3: n=24, Mean(pg/ml): 3497, Standard deviation: 202.8, CV(%): 5.8
Restrictions For Research Use only

Handling

Product Details VEGFC ELISA Kit Target details Application Details References for VEGFC Kit (ABIN411382) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for VEGFC Kit (ABIN411382)

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Product cited in:

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, 2015

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, 2015

Song, Yue, Li, Li, Zhao, Zhang: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, 2014

Zhang, Ding, Diao, Yan, Sun, Hu: "CYR61 modulates the vascular endothelial growth factor C expression of decidual NK cells via PI3K/AKT pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 3, pp. 216-23, 2012

Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." in: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, 2012

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, 2010

Wang, Zhao, Guo, Zhang, Xie, Xiang, Gao, Wang, Chen: "The expression of RNA-binding protein HuR in non-small cell lung cancer correlates with vascular endothelial growth factor-C expression and lymph node metastasis." in: Oncology, Vol. 76, Issue 6, pp. 420-9, 2009

Background publications

Zhao, Sun, Li: "Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 6, pp. 2873-7, 2012

Zhang, Zhang, Yao, Gao, Yang, Zhao, Rao, Gao: "M2-polarized macrophages promote metastatic behavior of Lewis lung carcinoma cells by inducing vascular endothelial growth factor-C expression." in: Clinics (São Paulo, Brazil), Vol. 67, Issue 8, pp. 901-6, 2012

Zhang, Wang, Gao, Guo, Chen, Wang, Gao, Rao, Chen: "Alternatively activated RAW264.7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma." in: Journal of cellular biochemistry, Vol. 107, Issue 1, pp. 134-43, 2009

Lee, Gray, Yuan, Luoh, Avraham, Wood: "Vascular endothelial growth factor-related protein: a ligand and specific activator of the tyrosine kinase receptor Flt4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 5, pp. 1988-92, 1996

Images

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Supplier Images
ELISA image for VEGFC ELISA Kit (Vascular Endothelial Growth Factor C) (ABIN411382) Human VEGF-C PicoKine ELISA Kit standard curve