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|Application / Reactivity||Human|
|ELISA||24 ELISA Kits|
|Antigen||Vascular Endothelial Growth Factor C (VEGFC) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||62.5-4000 pg/mL|
|Minimum Detection Limit||62.5 pg/mL|
|11 references available|
|Supplier||Log in to see|
Product Details VEGFC ELISA KitTarget details Application Details Handling References for VEGFC Kit (ABIN411382) Images
|Purpose||For quantitative detection of human VEGF-C in cell culture supernates, serum and plasma(heparin, EDTA).|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)|
|Specificity||Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: T103-R227
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|Background||Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.|
|Research Area||Cancer, Angiogenesis, Growth Factors|
Application DetailsProduct Details VEGFC ELISA Kit Target details Handling References for VEGFC Kit (ABIN411382) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||human VEGF-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF-C has been precoated onto 96-well plates. Standards(NSO, T103-R227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGF-C amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 4000pg/mL,2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGF-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human VEGF-C standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details VEGFC ELISA Kit Target details Application Details References for VEGFC Kit (ABIN411382) Images back to top
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for VEGFC Kit (ABIN411382)Product Details VEGFC ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Xu, Ge, Han et al.: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, 2015 (PubMed).
Song, Yue, Li et al.: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, 2014 (PubMed).
Li, Wang, Zhao et al.: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, 2015 (PubMed).
Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." in: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, 2012 (PubMed).
Zhang, Ding, Diao et al.: "CYR61 modulates the vascular endothelial growth factor C expression of decidual NK cells via PI3K/AKT pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 3, pp. 216-23, 2012 (PubMed).
Yang, Shi, Li et al.: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, 2010 (PubMed).
Wang, Zhao, Guo et al.: "The expression of RNA-binding protein HuR in non-small cell lung cancer correlates with vascular endothelial growth factor-C expression and lymph node metastasis." in: Oncology, Vol. 76, Issue 6, pp. 420-9, 2009 (PubMed).
Zhang, Zhang, Yao et al.: "M2-polarized macrophages promote metastatic behavior of Lewis lung carcinoma cells by inducing vascular endothelial growth factor-C expression." in: Clinics (São Paulo, Brazil), Vol. 67, Issue 8, pp. 901-6, 2012 (PubMed).
Zhao, Sun, Li: "Expression and clinical significance of STAT3, P-STAT3, and VEGF-C in small cell lung cancer." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 6, pp. 2873-7, 2012 (PubMed).
Zhang, Wang, Gao et al.: "Alternatively activated RAW264.7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma." in: Journal of cellular biochemistry, Vol. 107, Issue 1, pp. 134-43, 2009 (PubMed).
Lee, Gray, Yuan et al.: "Vascular endothelial growth factor-related protein: a ligand and specific activator of the tyrosine kinase receptor Flt4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 5, pp. 1988-92, 1996 (PubMed).
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