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|Antigen||Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies|
|Epitope||C-Term, AA 1158-1365 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This VEGFR2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|8 references available|
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Product Details anti-VEGFR2 AntibodyTarget Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) Images
|Immunogen||Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]|
Target Details VEGFR2Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) Images back to top
|Alternative Name||CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)|
|Background||Gene Symbol: KDR|
|Molecular Weight||Theoretical MW: 150 kDa|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling|
Application DetailsProduct Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN152058) Images back to top
|Application Notes||Western Blot 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 2-5 μL, Immunohistochemistry-Paraffin 1:10-1:500This VEGF Receptor 2 antibody is useful for Immunohistochemistry paraffin-embedded sections (PMID 10751359), Immunoprecipitation and Western blot where a doublet can been seen at ~ 150 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 20 µg of total transfected protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS with 0.5 % BSA for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN152058) Images back to top
Buffer contains: 0.1 % Sodium Azide
|Precaution of Use|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-VEGFR2 antibody (ABIN152058)Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
|Product cited in:||
Ying, Wang, Yang, Gu, Mai, Qiu, Chen, Wang: "Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway." in: Life sciences, Vol. 144, pp. 208-17, 2016 (Sample species: Human). Further details: Western Blotting
Tsuboi, Yamashita, Nagano, Kimura, Toa Salazar, Ohneda: "Impaired expression of HIF-2α induces compensatory expression of HIF-1α for the recovery from anemia." in: Journal of cellular physiology, Vol. 230, Issue 7, pp. 1534-48, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
André, Tunik, Aronsson, Kvanta: "Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model." in: Investigative ophthalmology & visual science, Vol. 56, Issue 11, pp. 6591-604, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008
Singh, Meyer, Band, Rahimi: "The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 2106-18, 2005 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation
Meyer, Singh, Rahimi: "The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling." in: The Journal of biological chemistry, Vol. 279, Issue 1, pp. 735-42, 2003 Further details: Western Blotting,Immunoprecipitation
Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation
Lashkari, Hirose, Yazdany, McMeel, Kazlauskas, Rahimi: "Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity." in: The American journal of pathology, Vol. 156, Issue 4, pp. 1337-44, 2000 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
ImagesProduct Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) back to top