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Human VEGFR2 ELISA Kit

Recommended VEGFR2 Product (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ELISA Kits
  • 6130401C07
  • CD309
  • fk52c05
  • flk
  • FLK-1
  • flk-1
  • Flk-1
  • FLK/KDR
  • Flk1
  • flk1
  • FLK1
  • flk1b
  • kdr
  • KDR
  • kdra
  • kdrb
  • Krd-1
  • Ly73
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • sVEGFR-2
  • VEGFR
  • Vegfr-2
  • vegfr-2
  • VEGFR-2
  • VEGFR2
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fc31a09
  • wu:fk52c05
Epitope
AA 20-764
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
31
24
19
6
4
3
3
3
2
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1672753
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414630 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.62-40 ng/mL 0.62 ng/mL Log in to see 3
0.30628014 ABIN2708412 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.30628014 ABIN2115157 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012155735 ABIN365817 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.39-25 ng/mL 0.39 ng/mL Log in to see 1
0.0012155735 ABIN626515 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1-25 ng/mL 1 ng/mL Log in to see
0.0012155735 ABIN3198628 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Log in to see
0.0012155735 ABIN1030145 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.625-40 ng/mL 0.625 ng/mL Log in to see
0.0012155735 ABIN1380731 Cell ELISA Log in to see
0.0012155735 ABIN1380732 Cell ELISA Log in to see
0.0012155735 ABIN1380730 Cell ELISA Log in to see
0.0012155735 ABIN1380277 Cell ELISA Log in to see
0.0012155735 ABIN809104 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012155735 ABIN1381587 Cell ELISA Log in to see
0.0012155735 ABIN2042723 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2684783 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Samples, Urine 30-800 pg/mL 30 pg/mL Log in to see
0.0012155735 ABIN2684784 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2113917 Cell ELISA Log in to see
0.0012155735 ABIN2113916 Cell ELISA Log in to see
0.0012155735 ABIN2113918 Cell ELISA Log in to see
0.0012155735 ABIN2113915 Cell ELISA Log in to see

Top referenced VEGFR2 ELISA Kit for ELISA

  • Human VEGFR2 ELISA Kit for Sandwich ELISA - ABIN414630 (3 Publications): Nassehi, Sørensen, Dyrbye, Thomsen, Juhler, Laursen, Broholm: Peritumoral brain edema in angiomatous supratentorial meningiomas: an investigation of the vascular endothelial growth factor A pathway. in APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 (PubMed)

  • Human VEGFR2 ELISA Kit for Sandwich ELISA - ABIN1672753 (6 Publications): Li, Huang, Chen, Chen, Xiong, Chen, You, Jin, Liang: Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction. in Journal of biomedical materials research. Part A 2010 (PubMed)

  • Human VEGFR2 ELISA Kit for Sandwich ELISA - ABIN365817 (1 Publications): Du, Wang, Ren, Lv, He: Revealing multi-binding sites for taspine to VEGFR-2 by cell membrane chromatography zonal elution. in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2012 (PubMed)

Similar VEGFR2 ELISA Kits

Application / Reactivity Human
ELISA 32 ELISA Kits

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ELISA Kits
Epitope AA 20-764
Reactivity Human
Kits with alternative reactivity to:
(31), (24), (19), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details VEGFR2 ELISA Kit

Target details Application Details Handling References for VEGFR2 Kit (ABIN1672753) Images
Purpose For quantitative detection of human VEGFR2 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human VEGFR2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <4pg/mL
Components
  • 96-well plate precoated with anti- human VEGFR2 antibod - 1
  • Lyophilized recombinant human VEGFR2 standar - 10ng/tubex2
  • Biotinylated anti- human VEGFR2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A20-E764

Target details

Product Details VEGFR2 ELISA Kit Application Details Handling References for VEGFR2 Kit (ABIN1672753) Images back to top
Antigen
Alternative Name KDR (KDR ELISA Kit Abstract)
Background Vascular endothelial growth factor receptor 2(VEGFR-2) also known as Kinase insert domain receptor(KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
Gene ID 3791
UniProt P35968
Pathways RTK Signaling

Application Details

Product Details VEGFR2 ELISA Kit Target details Handling References for VEGFR2 Kit (ABIN1672753) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E764) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGFR2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human VEGFR2 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 197, Standard deviation: 9.65, CV(%): 4.9
  • Sample 2: n=16, Mean(pg/ml): 1466, Standard deviation: 51.31, CV(%): 3.5
  • Sample 3: n=16, Mean(pg/ml): 2458, Standard deviation: 127.8, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 234, Standard deviation: 17.55, CV(%): 7.5
  • Sample 2: n=24, Mean(pg/ml): 1630, Standard deviation: 114.1, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 2863, Standard deviation: 177.5, CV(%): 6.2
Restrictions For Research Use only

Handling

Product Details VEGFR2 ELISA Kit Target details Application Details References for VEGFR2 Kit (ABIN1672753) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for VEGFR2 Kit (ABIN1672753)

Product Details VEGFR2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, 2013

Background publications

Li, Li, Xu, Tang, Gao, Xu, Liu, Xie, Tu, Peng, Qiu, Liang: "Effects of anti-rVEGF on the expression of VEGF receptor-2 and P2X(2/3) receptors of the spinal dorsal horn in neuropathic pain rats." in: Brain research bulletin, Vol. 87, Issue 2-3, pp. 227-33, 2012

Zhou, Zhan, Zhou, Li, Lin, Tang, Shu, Chen, Zeng, Cai, Jiang: "A study of the distribution and density of the VEGFR-2 receptor on glioma microvascular endothelial cell membranes." in: Cellular and molecular neurobiology, Vol. 31, Issue 5, pp. 687-94, 2011

Li, Huang, Chen, Chen, Xiong, Chen, You, Jin, Liang: "Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction." in: Journal of biomedical materials research. Part A, Vol. 94, Issue 4, pp. 1283-93, 2010

Shay-Salit, Shushy, Wolfovitz, Yahav, Breviario, Dejana, Resnick: "VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9462-7, 2002

Sait, Dougher-Vermazen, Shows, Terman: "The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-->q12." in: Cytogenetics and cell genetics, Vol. 70, Issue 1-2, pp. 145-6, 1995

Images

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Supplier Images
ELISA image for VEGFR2 ELISA Kit (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (ABIN1672753) Human VEGFR2/KDR PicoKine ELISA Kit standard curve