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|Application / Reactivity||Human|
|ELISA||32 ELISA Kits|
|Antigen||Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||62.5-4000 pg/mL|
|Minimum Detection Limit||62.5 pg/mL|
|6 references available|
|Supplier||Log in to see|
Product Details VEGFR2 ELISA KitTarget details Application Details Handling References for VEGFR2 Kit (ABIN1672753) Images
|Purpose||For quantitative detection of human VEGFR2 in cell culture supernates, serum and plasma(heparin, EDTA).|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)|
|Specificity||Natural and recombinant human VEGFR2|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: A20-E764
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|Background||Vascular endothelial growth factor receptor 2(VEGFR-2) also known as Kinase insert domain receptor(KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.|
Application DetailsProduct Details VEGFR2 ELISA Kit Target details Handling References for VEGFR2 Kit (ABIN1672753) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E764) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGFR2 amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human VEGFR2 standard solution and each sample is measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details VEGFR2 ELISA Kit Target details Application Details References for VEGFR2 Kit (ABIN1672753) Images back to top
|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for VEGFR2 Kit (ABIN1672753)Product Details VEGFR2 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Wang, Liu, Wang et al.: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, 2013 (PubMed).
Li, Li, Xu et al.: "Effects of anti-rVEGF on the expression of VEGF receptor-2 and P2X(2/3) receptors of the spinal dorsal horn in neuropathic pain rats." in: Brain research bulletin, Vol. 87, Issue 2-3, pp. 227-33, 2012 (PubMed).
Zhou, Zhan, Zhou et al.: "A study of the distribution and density of the VEGFR-2 receptor on glioma microvascular endothelial cell membranes." in: Cellular and molecular neurobiology, Vol. 31, Issue 5, pp. 687-94, 2011 (PubMed).
Li, Huang, Chen et al.: "Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction." in: Journal of biomedical materials research. Part A, Vol. 94, Issue 4, pp. 1283-93, 2010 (PubMed).
Shay-Salit, Shushy, Wolfovitz et al.: "VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9462-7, 2002 (PubMed).
Sait, Dougher-Vermazen, Shows et al.: "The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-->q12." in: Cytogenetics and cell genetics, Vol. 70, Issue 1-2, pp. 145-6, 1995 (PubMed).
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