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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||5 Antibodies||9 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||5 Antibodies|
|Western Blotting (WB)||24 Antibodies||32 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Antigen||Wiskott-Aldrich Syndrome-Like (WASL) Antibodies|
|Epitope||AA 129-156, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This WASL antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-WASL AntibodyTarget Details WASL Application Details Handling Images
|Sequence||ADEDEAQAFR ALVQEKIQKR NQRQSGDR|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Wiskott-Aldrich syndrome protein(WAS) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: Wiskott-Aldrich syndrome
Protein Name: Wiskott-Aldrich syndrome protein
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human WASP (129-156aa ADEDEAQAFRALVQEKIQKRNQRQSGDR), different from the related mouse sequence by two amino acids.|
Target Details WASLProduct Details anti-WASL Antibody Application Details Handling Images back to top
|Alternative Name||WAS (WASL Antibody Abstract)|
|Background||The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.|
|Pathways||RTK Signaling, TCR Signaling|
Application DetailsProduct Details anti-WASL Antibody Target Details WASL Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for WASP is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-WASL Antibody Target Details WASL Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-WASL Antibody Target Details WASL Application Details Handling back to top