anti-Rat (Rattus) AIF1 antibody for Immunocytochemistry

Recommended AIF1 Antibody (supplied by: Log in to see )

Antigen
Allograft Inflammatory Factor 1 (AIF1) Antibodies
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • C9ORF58
Epitope
C-Term, Isoform 1
36
25
20
15
14
14
10
8
7
6
4
4
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
212
106
97
8
7
2
2
1
1
Host
Goat
142
55
37
2
1
Clonality
Polyclonal
Conjugate
This AIF1 antibody is un-conjugated
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm)), Western Blotting (WB)
183
122
105
67
27
26
19
13
10
10
7
4
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN249515
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4278832 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 1
1 ABIN2855067 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278831 ICC IF IHC IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1175634 ICC IHC IP WB Rabbit IgG AA 1-147 Log in to see Polyclonal
1 ABIN3201704 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 1-147 Log in to see
1 ABIN4278827 ELISA ICC IF IHC IHC (ff) IHC (fro) IHC (p) IHC (wm) WB Goat C-Term, Isoform 1 Log in to see Polyclonal
1 ABIN4278835 FACS ICC IF IHC (p) WB DyLight 405LS Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278834 FACS ICC IF IHC (p) WB DyLight 405 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278836 FACS ICC IF IHC (p) WB DyLight 488 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278837 FACS ICC IF IHC (p) WB DyLight 550 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278840 FACS ICC IF IHC (p) WB DyLight 755 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278843 FACS ICC IF IHC (p) WB Alexa Fluor 647 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278845 ICC IF IHC (p) WB Biotin Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278846 FACS ICC IF IHC (p) FITC Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278838 FACS ICC IF IHC (p) WB DyLight 650 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278839 FACS ICC IF IHC (p) WB DyLight 680 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278842 FACS ICC IF IHC (p) WB Alexa Fluor 488 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278833 FACS ICC IF IHC (p) WB DyLight 350 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278841 FACS ICC IF IHC (p) WB Alexa Fluor 405 Rabbit IgG Center Log in to see Polyclonal
1 ABIN4278844 FACS ICC IF IHC (p) WB Alexa Fluor 700 Rabbit IgG Center Log in to see Polyclonal

General

Antigen Allograft Inflammatory Factor 1 (AIF1) Antibodies
Epitope C-Term, Isoform 1
(36), (25), (20), (15), (14), (14), (10), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(212), (106), (97), (8), (7), (2), (2), (1), (1)
Host Goat
(142), (55), (37), (2), (1)
Clonality
Polyclonal
Conjugate This AIF1 antibody is un-conjugated
(23), (22), (11), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm)), Western Blotting (WB)
(183), (122), (105), (67), (27), (26), (19), (13), (10), (10), (7), (4), (2), (2), (1), (1), (1)
Pubmed 20 references available
Supplier Log in to see

Product Details anti-AIF1 Antibody

Target Details AIF1 Application Details Handling References for anti-AIF1 antibody (ABIN249515) Images
Specificity This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF1/ IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
Purification Immunogen affinity purified
Immunogen Peptide with sequence C-TGPPAKKAISELP corresponding to C-Terminus according to NP_001614.3.

Target Details AIF1

Product Details anti-AIF1 Antibody Application Details Handling References for anti-AIF1 antibody (ABIN249515) Images back to top
Antigen
Alternative Name AIF-1/Iba1 (AIF1 Antibody Abstract)
Background Gene Symbol: AIF1
Molecular Weight Theoretical MW: 17 kDa
Gene ID 199
UniProt P55008
Pathways Smooth Muscle Cell Migration

Application Details

Product Details anti-AIF1 Antibody Target Details AIF1 Handling References for anti-AIF1 antibody (ABIN249515) Images back to top
Application Notes Western Blot 0.2-1 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 2-4 μg/mL, Immunohistochemistry-Frozen 1:10-1:500, Peptide ELISA Detection Limit 1:32000, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, ImmunocytochemistryWestern blot: Approx 16 kDa band observed in Rat Brain lysates (calculated MW of 16.7 kDa according to NP_001614.3). IHC: In paraffin embedded Mouse Brain shows distinct staining of astrocytes. Data obtained from a previous batch (different goat).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AIF1 Antibody Target Details AIF1 Application Details References for anti-AIF1 antibody (ABIN249515) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-AIF1 antibody (ABIN249515)

Product Details anti-AIF1 Antibody Target Details AIF1 Application Details Handling Images back to top
Product cited in:

Kim, Hwang, Lee, Kim, Son, Ham, Hellström, Han, Kim, Park, Hong: "Inhibitory effect of punicalagin on lipopolysaccharide-induced neuroinflammation, oxidative stress and memory impairment via inhibition of nuclear factor-kappaB." in: Neuropharmacology, Vol. 117, pp. 21-32, 2017 Method employed by authors: Immunohistochemistry (IHC), Western Blotting (WB) (Sample species: Mouse (Murine)).

Sagy-Bross, Kasianov, Solomonov, Braiman, Friedman, Hadad, Levy: "The role of cytosolic phospholipase A2 α in amyloid precursor protein induction by amyloid beta1-42 : implication for neurodegeneration." in: Journal of neurochemistry, Vol. 132, Issue 5, pp. 559-71, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Zeng, Garg, Mach: "The PGRMC1 Protein Level Correlates with the Binding Activity of a Sigma-2 Fluorescent Probe (SW120) in Rat Brain Cells." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 18, Issue 2, pp. 172-9, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Hwang, Park, Choi, Choi, Oh, Hwang, Han, Hong: "Acceleration of amyloidogenesis and memory impairment by estrogen deficiency through NF-κB dependent beta-secretase activation in presenilin 2 mutant mice." in: Brain, behavior, and immunity, Vol. 53, pp. 113-22, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Chakravarthy, Navitskaya, OReilly, Gallimore, Mize, Beli, Wang, Kady, Huang, Blanchard, Grant, Busik: "Role of Acid Sphingomyelinase in Shifting the Balance Between Proinflammatory and Reparative Bone Marrow Cells in Diabetic Retinopathy." in: Stem cells (Dayton, Ohio), Vol. 34, Issue 4, pp. 972-83, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Whole Mount)

Bhandare, Kapoor, Pilowsky, Farnham: "Seizure-Induced Sympathoexcitation Is Caused by Activation of Glutamatergic Receptors in RVLM That Also Causes Proarrhythmogenic Changes Mediated by PACAP and Microglia in Rats." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 2, pp. 506-17, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Free Floating)

Kapoor, Bhandare, Nedoboy, Mohammed, Farnham, Pilowsky: "Dynamic changes in the relationship of microglia to cardiovascular neurons in response to increases and decreases in blood pressure." in: Neuroscience, Vol. 329, pp. 12-29, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Free Floating)

Solomonov, Hadad, Levy: "Reduction of cytosolic phospholipase A2α upregulation delays the onset of symptoms in SOD1G93A mouse model of amyotrophic lateral sclerosis." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 134, 2016 Further details: Immunohistochemistry (Frozen Sections)

Kim, Lew, Spirkina, Mizuno: "Xenin-induced feeding suppression is not mediated through the activation of central extracellular signal-regulated kinase signaling in mice." in: Behavioural brain research, Vol. 312, pp. 118-26, 2016

Raspollini, Luque, Menendez, Bollito, Brunelli, Martignoni, Montironi, Cheng, Blanca, Baroni, Minervini, Lopez-Beltran: "T1 high-grade bladder carcinoma outcome: the role of p16, topoisomerase-IIα, survivin, and E-cadherin." in: Human pathology, Vol. 57, pp. 78-84, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Rodhe, Burguillos, de Pablos, Kavanagh, Persson, Englund, Deierborg, Venero, Joseph: "Spatio-temporal activation of caspase-8 in myeloid cells upon ischemic stroke." in: Acta neuropathologica communications, Vol. 4, Issue 1, pp. 92, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Chakravarthy, Beli, Navitskaya, OReilly, Wang, Kady, Huang, Grant, Busik: "Imbalances in Mobilization and Activation of Pro-Inflammatory and Vascular Reparative Bone Marrow-Derived Cells in Diabetic Retinopathy." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146829, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Choi, Ahn, Yang, Kim, Chong, Kim: "Hippocampus-based contextual memory alters the morphological characteristics of astrocytes in the dentate gyrus." in: Molecular brain, Vol. 9, Issue 1, pp. 72, 2016 Method employed by authors: Western Blotting (WB)

Loewen, Barker-Haliski, Dahle, White, Wilcox: "Neuronal Injury, Gliosis, and Glial Proliferation in Two Models of Temporal Lobe Epilepsy." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 4, pp. 366-78, 2016

Theus, Sparks, Liao, Ren, Luo: "All- Trans-Retinoic Acid Augments the Histopathological Outcome of Neuroinflammation and Neurodegeneration in Lupus-Prone MRL/lpr Mice." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 65, Issue 2, pp. 69-81, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Roche, Wyse-Jackson, Gómez-Vicente, Lax, Ruiz-Lopez, Byrne, Cuenca, Cotter: "Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling." in: PLoS ONE, Vol. 11, Issue 11, pp. e0165197, 2016 (Sample species: Human).

Zhan, Qin, Zhang, Jiang, Ma, Li, Luo et al.: "Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in ..." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 258, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Côté, Poirier, Aubé, Jobin, Lacroix, Soulet: "Partial depletion of the proinflammatory monocyte population is neuroprotective in the myenteric plexus but not in the basal ganglia in a MPTP mouse model of Parkinson's disease." in: Brain, behavior, and immunity, Vol. 46, pp. 154-67, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Xie, Wang, Zhou, Li, Zhang, Zhou, Zhang, Hang, Zhou, Shi: "Expression of Cytoplasmic Gelsolin in Rat Brain After Experimental Subarachnoid Hemorrhage." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 723-31, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Aniszewska, Chłodzińska, Bartkowska, Winnicka, Turlejski, Djavadian: "The expression of interleukin-6 and its receptor in various brain regions and their roles in exploratory behavior and stress responses." in: Journal of neuroimmunology, Vol. 284, pp. 1-9, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Images

Product Details anti-AIF1 Antibody Target Details AIF1 Application Details Handling References for anti-AIF1 antibody (ABIN249515) back to top
Supplier Images
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) ABIN249515 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Pri...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Western Blot: AIF-1/Iba1 Antibody [ABIN249515] - (1.5ug/ml) staining of Rat Brain lys...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Western Blot: AIF-1/Iba1 Antibody [ABIN249515] - Staining of Human Frontal Cortex (A)...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [ABIN249515] - (2ug/ml) staining o...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [ABIN249515] - Staining of frozen ra...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [ABIN249515] - Iba-1 in mouse brai...
Immunocytochemistry (ICC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Immunocytochemistry: AIF-1/Iba1 Antibody [ABIN249515] - Analysis of Iba1 in rat sampl...
Immunocytochemistry (ICC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Immunocytochemistry: AIF-1/Iba1 Antibody [ABIN249515] - Analysis of Iba1 in rat brain...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term), (Isoform 1) antibody (ABIN249515) Western Blot: AIF-1/Iba1 Antibody - Staining of Human (A) and (0.5ug/ml) of Mouse (B...