Plant Physiology (June '09)

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Estimate subcellular fraction purity with markers for cell compartments

Here you may find a set of antibodies against plant cell compartments. These antibodies are useful in estimating subcellular fraction purity:

Organelle Antibody
Cell wall

Xet antibody

XTH-Xet (Xet5) antibody

Chloroplast Ascorbate oxidase (Higher Plants) antibody
Chloroplast inner envelope membrane Tic (chloroplast envelope protein) (Higher Plants) antibody
Chloroplast outer envelope membrane Toc (chloroplast envelope protein) (Higher Plants) antibody
Chloroplast Plastoglobules AtPGL35 antibody
Chloroplast thylakoid and envelop membranes CRD1 antibody
Chloroplast thylakoid lumen Plastocyanin (PC) antibody
Cytoplasm

cFBPase antibody

Hsc70 (Wheat Germ) antibody

Maize SPS (Higher Plants) antibody

SPS (Sucrose phosphate synthase) antibody

UGPase antibody

Endoplasmatic reticulum Sar1 (secretion-associated and Ras-related protein 1) antibody
Golgi

Arf1 (ADP-ribosylation factor 1) antibody

Sec21 (gamma subunit, COP vesicles) (Golgi marker) antibody

Mitochondrial inner membrane

AOX (Chlamydomonas) antibody

COXII (Chlamydomonas) antibody

Mitochondrial matrix

GDC H antibody

Idh (isocytrate dehydrogenase) (Higher Plants) antibody

SHMT antibody

Mitochondrial outer membrane VDAC1 (outer mitochondrial membrane) (Higher Plants ) antibody
Nucleoplasm At3g25940 (transcription factor S-II (TFIIS) domain containing protein antibody
Plasma Membrane H+ATPase (plant plasma membrane marker) antibody
Plastid stroma Hcf101 antibody
Plastid stroma in higher plants and cytoplasm in cyanobacteria RbcL (Type I + Type II) antibody
Thylakoid lumen cyt c6 antibody
Thylakoid membrane

PsbA (D1) antibody

PsbE antibody

Tonoplast membrane V-ATPase (tonoplast marker) (Higher Plants ) antibody