Plant Physiology (June '09)

Estimate subcellular fraction purity with markers for cell compartments

Here you may find a set of antibodies against plant cell compartments. These antibodies are useful in estimating subcellular fraction purity:

Organelle	Antibody
Cell wall	Xet antibody XTH-Xet (Xet5) antibody
Chloroplast	Ascorbate oxidase (Higher Plants) antibody
Chloroplast inner envelope membrane	Tic (chloroplast envelope protein) (Higher Plants) antibody
Chloroplast outer envelope membrane	Toc (chloroplast envelope protein) (Higher Plants) antibody
Chloroplast Plastoglobules	AtPGL35 antibody
Chloroplast thylakoid and envelop membranes	CRD1 antibody
Chloroplast thylakoid lumen	Plastocyanin (PC) antibody
Cytoplasm	cFBPase antibody Hsc70 (Wheat Germ) antibody Maize SPS (Higher Plants) antibody SPS (Sucrose phosphate synthase) antibody UGPase antibody
Endoplasmatic reticulum	Sar1 (secretion-associated and Ras-related protein 1) antibody
Golgi	Arf1 (ADP-ribosylation factor 1) antibody Sec21 (gamma subunit, COP vesicles) (Golgi marker) antibody
Mitochondrial inner membrane	AOX (Chlamydomonas) antibody COXII (Chlamydomonas) antibody
Mitochondrial matrix	GDC H antibody Idh (isocytrate dehydrogenase) (Higher Plants) antibody SHMT antibody
Mitochondrial outer membrane	VDAC1 (outer mitochondrial membrane) (Higher Plants ) antibody
Nucleoplasm	At3g25940 (transcription factor S-II (TFIIS) domain containing protein antibody
Plasma Membrane	H+ATPase (plant plasma membrane marker) antibody
Plastid stroma	Hcf101 antibody
Plastid stroma in higher plants and cytoplasm in cyanobacteria	RbcL (Type I + Type II) antibody
Thylakoid lumen	cyt c6 antibody
Thylakoid membrane	PsbA (D1) antibody PsbE antibody
Tonoplast membrane	V-ATPase (tonoplast marker) (Higher Plants ) antibody