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|Antigen||Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies|
|Epitope||AA 595-810 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CBL antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-CBL AntibodyTarget Details CBL Application Details Handling References for anti-CBL antibody (ABIN967970) Images
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human c-Cbl aa. 595-810|
Target Details CBLProduct Details anti-CBL Antibody Application Details Handling References for anti-CBL antibody (ABIN967970) Images back to top
|Alternative Name||c-Cbl (CBL Antibody Abstract)|
|Background||Cbl was identified in the genome of a transforming retrovirus from a mouse pre-B cell lymphoma. The product of mammalian c-cbl, p120[cbl], is a widely expressed cytoplasmic protein with several distinctive domains including a RING finger motif and a large proline-rich domain. Although the normal cellular function of c-Cbl has not yet been determined, it associates with many well known signal transduction proteins. In lymphoid cells, c-Cbl is a substrate of TCR-activated protein tyrosine kinases. In addition, stimulation with EGF, PDGF, FGF, or NGF results in tyrosine-phosphorylation of c-Cbl in lymphoid and other cell types. It forms a signaling complex with Grb2 in T cells and is a SH3 and SH2 binding protein which associates with many other cytoplasmic signaling proteins including Btk, Src, fyn, lck, and the beta isoform of PI-3 kinase. Thus, c-Cbl may be an important adaptor protein in growth factor and T-cell receptor signaling pathways. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||120 kDa|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-CBL Antibody Target Details CBL Handling References for anti-CBL antibody (ABIN967970) Images back to top
Related Products: ABIN968537, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-CBL Antibody Target Details CBL Application Details References for anti-CBL antibody (ABIN967970) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-CBL antibody (ABIN967970)Product Details anti-CBL Antibody Target Details CBL Application Details Handling Images back to top
|Product cited in:||
Jiang, Huang, Marusyk, Sorkin: "Grb2 regulates internalization of EGF receptors through clathrin-coated pits." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 858-70, 2003
Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, 2001
Garcia, Miller: "Single-cell analyses reveal two defects in peptide-specific activation of naive T cells from aged mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3151-7, 2001
Sanjay, Houghton, Neff, DiDomenico, Bardelay, Antoine, Levy, Gailit, Bowtell, Horne, Baron: "Cbl associates with Pyk2 and Src to regulate Src kinase activity, alpha(v)beta(3) integrin-mediated signaling, cell adhesion, and osteoclast motility." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 181-95, 2001
Donovan, Wange, Langdon, Samelson: "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen receptor." in: The Journal of biological chemistry, Vol. 269, Issue 37, pp. 22921-4, 1994
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