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anti-Human CBL antibody for Intracellular Staining

Recommended CBL Antibody (supplied by: Log in to see )

Antigen
Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
  • 4732447J05Rik
  • C-CBL
  • c-Cbl
  • c-cbl-like
  • cbl
  • Cbl-2
  • CBL2
  • FRA11B
  • NSLL
  • RGD1561386
  • RNF55
Epitope
pTyr700
46
39
26
23
17
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
249
134
129
6
4
4
4
1
1
1
Host
Mouse
202
35
16
1
Clonality
Monoclonal
Conjugate
This CBL antibody is conjugated to Alexa Fluor 647
7
7
7
6
6
5
5
5
5
5
5
5
4
2
2
Application
Intracellular Staining (ICS)
169
71
67
55
55
32
18
17
6
4
4
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN1176885
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086434494 ABIN1176886 ICS Alexa Fluor 488 Mouse IgG1 pTyr700 Log in to see 47-c 3
0.00086434494 ABIN1177043 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr774 Log in to see 29-c 2
0.00086434494 ABIN1177042 ICS PE Mouse IgG1, kappa pTyr774 Log in to see 29-c 2
0.00086434494 ABIN1176884 ICS PE Mouse IgG1 pTyr700 Log in to see 47-c 3

Top referenced anti-CBL antibody for Intracellular Staining

Similar anti-CBL Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 168 Antibodies 79 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 5 Antibodies 3 Antibodies
Immunoprecipitation (IP) 20 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 72 Antibodies 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies 55 Antibodies
Immunofluorescence (IF) 55 Antibodies 27 Antibodies
Immunocytochemistry (ICC) 17 Antibodies 6 Antibodies

General

Antigen Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
Epitope pTyr700
(46), (39), (26), (23), (17), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(249), (134), (129), (6), (4), (4), (4), (1), (1), (1)
Host Mouse
(202), (35), (16), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CBL antibody is conjugated to Alexa Fluor 647
(7), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2)
Application Intracellular Staining (ICS)
(169), (71), (67), (55), (55), (32), (18), (17), (6), (4), (4), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CBL Antibody

Target Details CBL Application Details Handling References for anti-CBL Antibody (ABIN1176885) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human c-Cbl (pY700) Peptide
Clone 47-c
Isotype IgG1

Target Details CBL

Product Details anti-CBL Antibody Application Details Handling References for anti-CBL Antibody (ABIN1176885) Images back to top
Antigen
Alternative Name c-Cbl (CBL Antibody Abstract)
Background Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700 (Y700), 731, and 774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y700 site interacts with the SH2 domains of CRK and Vav1. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 47/c-Cbl monoclonal antibody recognizes the Y700-phosphorylated form of human c-Cbl. The orthologous phosphorylation site in mouse c-Cbl is Y698.
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CBL Antibody Target Details CBL Handling References for anti-CBL Antibody (ABIN1176885) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-CBL Antibody Target Details CBL Application Details References for anti-CBL Antibody (ABIN1176885) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-CBL Antibody (ABIN1176885)

Product Details anti-CBL Antibody Target Details CBL Application Details Handling Images back to top
Product cited in:

Miura-Shimura, Duan, Rao, Reddi, Shimura, Rottapel, Druker, Tsygankov, Band, Band: "Cbl-mediated ubiquitinylation and negative regulation of Vav." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38495-504, 2003

Thien, Langdon: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, 2001

Tsygankov, Teckchandani, Feshchenko, Swaminathan: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, 2001