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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||30 Antibodies||34 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||3 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||24 Antibodies||24 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||55 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||72 Antibodies|
|Antigen||Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CBL antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CBL AntibodyTarget Details CBL Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CBL protein. (region surrounding Gly694)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details CBLProduct Details anti-CBL Antibody Application Details Handling Images back to top
|Alternative Name||CBL / RNF55 (CBL Antibody Abstract)|
|Background||Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL ,Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4,11) or t(11,14) abnormalities.Synonyms: CBL2, Casitas B-lineage lymphoma proto-oncogene, E3 ubiquitin-protein ligase CBL, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, c-CBL|
|Molecular Weight||approx. 100, 120 kDa|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CBL Antibody Target Details CBL Handling Images back to top
ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CBL Antibody Target Details CBL Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-CBL Antibody Target Details CBL Application Details Handling back to top