We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||164 Antibodies||76 Antibodies||72 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Staining (ICS)||5 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||20 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies||21 Antibodies||21 Antibodies|
|Immunohistochemistry (IHC)||69 Antibodies||34 Antibodies||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||55 Antibodies|
|Antigen||Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CBL antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CBL AntibodyTarget Details CBL Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CBL protein. (region surrounding Ser668)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details CBLProduct Details anti-CBL Antibody Application Details Handling Images back to top
|Alternative Name||CBL / RNF55 (CBL Antibody Abstract)|
|Background||Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4,11) or t(11,14) abnormalities. Two proteins related to c-Cbl have been identified as Cbl-b (RING finger protein 56) and Cbl-3 (RING finger protein 57). Cbl-b has a proline-rich domain, a nuclear localization signal, a C3HC4 zinc finger and a putative leucine zipper. Cbl-b is expressed in normal and malignant mammary epithelial cells, various normal tissues and hematopoietic tissue and cell lines. Data suggests that Cbl-b encodes a protein that can interact with signal transduction proteins to regulate their function or be regulated by them.Synonyms: CBL2, Casitas B-lineage lymphoma proto-oncogene, E3 ubiquitin-protein ligase CBL, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, c-CBL|
|Molecular Weight||approx. 100, 120 kDa|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CBL Antibody Target Details CBL Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CBL Antibody Target Details CBL Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-CBL Antibody Target Details CBL Application Details Handling back to top