anti-Human CD137 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD137 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Antibodies
  • TNFRSF9
  • zgc:136557
  • 4-1BB
  • CD137
  • CDw137
  • ILA
  • A930040I11Rik
  • AA408498
  • AI325004
  • Cd137
  • Ly63
  • tumor necrosis factor receptor superfamily member 9
  • TNF receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9a
  • tumor necrosis factor receptor superfamily, member 9
  • TNFRSF9
  • tnfrsf9a
  • Tnfrsf9
Epitope
AA 115-165
22
14
7
5
5
4
3
3
3
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
216
127
20
15
14
10
3
2
2
2
2
2
Host
Rabbit
119
105
55
27
19
Clonality
Polyclonal
Conjugate
This CD137 antibody is un-conjugated
29
23
15
11
11
10
9
9
6
6
6
6
6
6
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
137
97
21
18
17
12
12
8
8
5
4
3
2
2
2
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN740445
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN740454 IHC (p) WB HRP Rabbit IgG AA 115-165 Log in to see Polyclonal
1 ABIN740447 IHC (p) WB Biotin Rabbit IgG AA 115-165 Log in to see Polyclonal
1 ABIN4901160 ELISA IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN2629449 FACS IF IHC (p) Mouse IgG1, kappa Log in to see BBK-2

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) Antibodies
Epitope AA 115-165
(22), (14), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(216), (127), (20), (15), (14), (10), (3), (2), (2), (2), (2), (2)
Host Rabbit
(119), (105), (55), (27), (19)
Clonality
Polyclonal
Conjugate This CD137 antibody is un-conjugated
(29), (23), (15), (11), (11), (10), (9), (9), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (137), (97), (21), (18), (17), (12), (12), (8), (8), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CD137 Antibody

Target Details CD137 Application Details Handling References for anti-CD137 antibody (ABIN740445) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human CD137
Isotype IgG

Target Details CD137

Product Details anti-CD137 Antibody Application Details Handling References for anti-CD137 antibody (ABIN740445) Images back to top
Antigen
Alternative Name CD137 (TNFRSF9 Antibody Abstract)
Background

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Subcellular location: Membrane bound, Cell membrane

Synonyms: ILA, 4-1BB, CD137, CDw137, Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9

Gene ID 3604
UniProt Q07011
Research Area Stem Cells, Hematopoietic Progenitors, Cytokines, Innate Immunity, CD Antigens, Apoptosis/Necrosis, Surface Receptors of Immune Cells

Application Details

Product Details anti-CD137 Antibody Target Details CD137 Handling References for anti-CD137 antibody (ABIN740445) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CD137 Antibody Target Details CD137 Application Details References for anti-CD137 antibody (ABIN740445) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CD137 antibody (ABIN740445)

Product Details anti-CD137 Antibody Target Details CD137 Application Details Handling Images back to top
Product cited in:

Claycombe, Vomhof-DeKrey, Garcia, Johnson, Uthus, Roemmich et al.: "Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low ..." in: The Journal of nutritional biochemistry, Vol. 31, pp. 113-21, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Daquinag, Tseng, Salameh, Zhang, Amaya-Manzanares, Dadbin, Florez, Xu, Tong, Kolonin: "Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development." in: Cell death and differentiation, 2014

Images

Product Details anti-CD137 Antibody Target Details CD137 Application Details Handling References for anti-CD137 antibody (ABIN740445) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 115-165) antibody (ABIN740445) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD137/TNFRSF9...
'Independent Validation' Badge
Antigen CD137
Lot Number AG05152084
Method validated Flow Cytometry
Positive Control rat adipose-derived mesenchymal stem cells
Negative Control cells stained with secondary antibody only
Notes

Passed. ABIN740445 specifically recognizes Cd137 in rat adipose-derived mesenchymal stem cells.

Primary Antibody ABIN740445
Secondary Antibody donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322)
Protocol
  • Grow rat adipose-derived mesenchymal stem cells in DMEM high glucose, GLUTAMAX, pyruvate supplemented (Gibco, 31966021, lot 1774921) supplemented with 10% fetal bovine serum (Gibco, 16000044, lot 1774921) 1% penicillin/streptomycin (Gibco, 15140122, lot 1759446), at 37°C and 5% CO2 in ml on a flask to 95% confluency.
  • Wash cells with PBS buffer.
  • Detach cells from the flask with trypsin-versene solution (Lonza, H3BE17-161E, lot RB35937) for 3min at 37°C and 5% CO2.
  • Centrifuge cells for at 400xg for 5min at RT, aspirate the supernatant.
  • Resuspend cells in 1mL of 1% BSA in PBS per 2x106 cells and incubate 20min at RT.
  • Incubate cells with primary rabbit anti-CD137 antibody (antibodies-online, ABIN740445, lot AG05152084) diluted 1:35 in 1% BSA in PBS buffer for 20min on ice. Include a negative control without primary antibody.
  • Wash cells 1x with PBS buffer and centrifuge them for 400xg for 5min at RT.
  • Resuspend cells in 175μl 1% BSA in PBS buffer.
  • Incubate cells with secondary donkey anti-rabbit Alexa Fluor 680 conjugated antibody (ThermoFisher Scientific, A10043, lot 1812322) diluted 1:200 in buffer for 15min in ice.
  • Wash cells 2xwith PBS buffer and centrifuge them for 400xg for 5min at RT.
  • Resuspend the pellet in 500μl PBS buffer.
  • Data acquisition in a BD FACSCanto II flow cytometer, equipped with two laser lines of 488nm and 633nm and two scattered light detectors (FSC and SSC). Detect fluorescence in PMTs with filters suitable for the fluorescence of the fluorophore AF680 (735LP and 780/60BP). Threshold is established in FSC. The data is saved in files in FCS format and analyzed offline with the FACSDiva software (BD).
Experimental Notes

ABIN740445 reveals a specific fluorescence signal in rat adipose-derived mesenchymal stem cells. This is the first time that flow cytometry is performed in this type of cells using this antibody. No signal was registered in the negative control cells.

Validation Images
Flow Cytometry validation image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 115-165) antibody (ABIN740445)

Flow cytometric analysis of cells stained with ABIN740445 and an AF680-conjugated ...