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Mouse (Murine) CD137 ELISA Kit

Recommended CD137 Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
  • 4-1BB
  • A930040I11Rik
  • AA408498
  • AI325004
  • CD137
  • Cd137
  • CDw137
  • ILA
  • Ly63
  • TNFRSF9
  • zgc:136557
Epitope
AA 24-187
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
16
7
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1889339
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3065253 ABIN2708445 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012187671 ABIN854619 Sandwich ELISA Serum, Plasma, Tissue Homogenate 1000-15.63 pg/mL 1000 pg/mL Log in to see
0.0012187671 ABIN3032793 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 6.1-1500 pg/mL 6.1 pg/mL Log in to see
0.0012187671 ABIN2702796 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 6-1.500 pg/mL 6 pg/mL Log in to see
0.0012187671 ABIN2685735 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012187671 ABIN1164422 Log in to see
0.0012187671 ABIN2952191 31.2-2000 pg/mL 31.2 pg/mL Log in to see

Top referenced CD137 ELISA Kit for ELISA

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Application / Reactivity Mouse (Murine)
ELISA 8 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) ELISA Kits
Epitope AA 24-187
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(16), (7), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details CD137 ELISA Kit

Target details Application Details Handling References for CD137 Kit (ABIN1889339) Images
Purpose For quantitative detection of mouse TNFRSF9 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse TNFRSF9
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse TNFRSF9 antibod - 1
  • Lyophilized recombinant mouse TNFRSF9 standar - 10ng/tubex2
  • Biotinylated anti- mouse TNFRSF9 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: V24-L187

Target details

Product Details CD137 ELISA Kit Application Details Handling References for CD137 Kit (ABIN1889339) Images back to top
Antigen
Alternative Name TNFRSF9 (TNFRSF9 ELISA Kit Abstract)
Background CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
Gene ID 21942
UniProt P20334
Pathways TCR Signaling

Application Details

Product Details CD137 ELISA Kit Target details Handling References for CD137 Kit (ABIN1889339) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TNFRSF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF9 has been precoated onto 96-well plates. Standards(NSO, V24-L187) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF9 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF9 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 319, Standard deviation: 12.76, CV(%): 4
  • Sample 2: n=16, Mean(pg/ml): 746, Standard deviation: 28.3, CV(%): 3.8
  • Sample 3: n=16, Mean(pg/ml): 1253, Standard deviation: 66.5, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 286, Standard deviation: 16.6, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 833, Standard deviation: 37.5, CV(%): 4.5
  • Sample 3: n=24, Mean(pg/ml): 1353, Standard deviation: 94.7, CV(%): 7
Restrictions For Research Use only

Handling

Product Details CD137 ELISA Kit Target details Application Details References for CD137 Kit (ABIN1889339) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CD137 Kit (ABIN1889339)

Product Details CD137 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Jang, Lee, Kim, Kim, Kwon: "Human 4-1BB (CD137) signals are mediated by TRAF2 and activate nuclear factor-kappa B." in: Biochemical and biophysical research communications, Vol. 242, Issue 3, pp. 613-20, 1998

Arch, Thompson: "4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappaB." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 558-65, 1998

Schwarz, Arden, Lotz: "CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies." in: Biochemical and biophysical research communications, Vol. 235, Issue 3, pp. 699-703, 1997

Images

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Supplier Images
ELISA image for CD137 ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 9) (ABIN1889339) Mouse TNFRSF9/4-1BB PicoKine ELISA Kit standard curve