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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||93 Antibodies|
|Functional Studies (Func)||2 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||24 Antibodies|
|Immunohistochemistry (IHC)||28 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||31 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Microcytotoxicity Assay (MCA)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||133 Antibodies|
|Antigen||CD247 Molecule (CD247) Antibodies|
|Epitope||AA 36-54 Alternatives|
|Conjugate||This CD247 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD247 AntibodyTarget Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN115429) Images
This antibody reacts with a 19 kDa protein.
Cellular Localization: Cell membrane
|Immunogen||A synthetic peptide corresponding to aa 36-54 aa of the human CD3 zeta protein.|
Target Details CD247Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN115429) Images back to top
|Alternative Name||CD247 / CD3Z (CD247 Antibody Abstract)|
|Background||CD3 consists of five invariant polypeptide chains that associate to form three dimmers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. Zeta chain consists of three consecutive copies of the same motif.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN115429) Images back to top
Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1: 50-1: 100 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN115429) Images back to top
|Buffer||10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD247 Antibody (ABIN115429)Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Luqmani, Ricketts, Ryall et al.: "Prediction of response to endocrine therapy in breast cancer using immunocytochemical assays for pS2, oestrogen receptor and progesterone receptor." in: International journal of cancer. Journal international du cancer, Vol. 54, Issue 4, pp. 619-23, 1993 (PubMed).
Pallud, Le Doussal, Pichon et al.: "Immunohistochemistry of pS2 in normal human breast and in various histological forms of breast tumours." in: Histopathology, Vol. 23, Issue 3, pp. 249-56, 1993 (PubMed).
Lipponen, Eskelinen: "Expression of pS2 protein in transitional cell bladder tumours." in: The Journal of pathology, Vol. 173, Issue 4, pp. 327-32, 1994 (PubMed).
Henry, Nicholson, Hennessy et al.: "Expression of the oestrogen regulated pNR-2 mRNA in human breast cancer: relation to oestrogen receptor mRNA levels and response to tamoxifen therapy." in: British journal of cancer, Vol. 61, Issue 1, pp. 32-8, 1990 (PubMed).
Henry, Bennett, Piggott et al.: "Expression of the pNR-2/pS2 protein in diverse human epithelial tumours." in: British journal of cancer, Vol. 64, Issue 4, pp. 677-82, 1991 (PubMed).