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anti-Mouse (Murine) CD247 antibody for Intracellular Staining

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Epitope
pTyr142
33
28
23
23
22
14
14
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
268
120
28
4
2
2
1
1
Host
Mouse
155
99
15
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is conjugated to PE
30
19
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Intracellular Staining (ICS)
199
137
107
57
47
45
34
21
12
8
2
2
1
1
Supplier
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Catalog No. ABIN1177044
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1177046 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr142 Log in to see K25-407-69 1
0.0008709465 ABIN1177045 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr142 Log in to see K25-407-69 1

Top referenced anti-CD247 antibody for Intracellular Staining

Similar anti-CD247 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 187 Antibodies 108 Antibodies 23 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 44 Antibodies 24 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 34 Antibodies 9 Antibodies 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies 5 Antibodies
Immunofluorescence (IF) 58 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope pTyr142
(33), (28), (23), (23), (22), (14), (14), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(268), (120), (28), (4), (2), (2), (1), (1)
Host Mouse
(155), (99), (15), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is conjugated to PE
(30), (19), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Intracellular Staining (ICS)
(199), (137), (107), (57), (47), (45), (34), (21), (12), (8), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1177044) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human CD3zeta Peptide
Clone K25-407-69
Isotype IgG2a, kappa

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN1177044) Images back to top
Antigen
Alternative Name CD247 (CD3z) (CD247 Antibody Abstract)
Background The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d. Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and zeta. The CD3 z polypeptide (CD3z) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3z is the primary intracellular signal transducing subunit. It contains three ITAMs (Immunoreceptor Tyrosine-based Activation Motifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa. The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3z (CD247).
Synonyms: CD3zeta
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN1177044) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN1177044) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-CD247 Antibody (ABIN1177044)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Alberola-Ila, Takaki, Kerner, Perlmutter: "Differential signaling by lymphocyte antigen receptors." in: Annual review of immunology, Vol. 15, pp. 125-54, 1997

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1177044) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD247 antibody (CD247 Molecule) (pTyr142) (PE) (ABIN1177044) anti-CD247 Molecule (CD247) (pTyr142) antibody (PE)