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anti-Mouse (Murine) CD247 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Reactivity
Human, Mouse (Murine)
189
68
28
4
2
2
1
Host
Rabbit
98
92
11
1
Clonality
Polyclonal
Conjugate
This CD247 antibody is un-conjugated
25
17
7
4
4
4
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
143
100
87
31
28
22
14
12
12
8
3
2
1
1
Supplier
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Catalog No. ABIN272006
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008948799 ABIN373195 IHC (p) IP Rabbit Log in to see Polyclonal

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 21 Antibodies 78 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 8 Antibodies
Flow Cytometry (FACS) 33 Antibodies 93 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 12 Antibodies
Immunofluorescence (IF) 7 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 56 Antibodies
Functional Studies (Func) 2 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human, Mouse (Murine)
(189), (68), (28), (4), (2), (2), (1)
Host Rabbit
(98), (92), (11), (1)
Clonality
Polyclonal
Conjugate This CD247 antibody is un-conjugated
(25), (17), (7), (4), (4), (4), (2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(143), (100), (87), (31), (28), (22), (14), (12), (12), (8), (3), (2), (1), (1)
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling Images
Specificity This antibody detects endogenous levels of CD3 ζprotein. (region surrounding Lys36)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD247 / CD3Z (CD247 Antibody Abstract)
Background The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and η chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ζ chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ζ chain ITAMs has been solved.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ
Molecular Weight approx. 20 kDa
Gene ID 919
NCBI Accession NP_000725
UniProt P20963
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling back to top
Supplier Images
 image for anti-CD247 antibody (CD247 Molecule) (ABIN272006) anti-CD247 Molecule (CD247) antibody