We are preparing the requested document. Please wait, this may take a while...!
|Antigen||CD247 Molecule (CD247) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CD247 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CD247 AntibodyTarget Details CD247 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of CD3 ζprotein. (region surrounding Lys36)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details CD247Product Details anti-CD247 Antibody Application Details Handling Images back to top
|Alternative Name||CD247 / CD3Z (CD247 Antibody Abstract)|
|Background||The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and η chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ζ chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ζ chain ITAMs has been solved.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ|
|Molecular Weight||approx. 20 kDa|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD247 Antibody Target Details CD247 Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD247 Antibody Target Details CD247 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-CD247 Antibody Target Details CD247 Application Details Handling back to top