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anti-Mouse (Murine) CD247 antibody for Immunoprecipitation

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • im:7156386
  • LOC100136514
  • CD3Z
  • LOC100136549
  • LOC100305023
  • CD247
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • CD3-ZETA
  • CD3H
  • CD3Q
  • T3Z
  • TCRZ
  • TCRzeta
  • CD247 antigen like
  • CD3zeta-2
  • CD3zeta-1
  • CD3 zeta
  • CD247 molecule
  • CD247 antigen
  • Cd247 molecule
  • cd247l
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • cd247
  • Cd247
Epitope
C-Term
34
22
16
16
14
14
12
6
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
244
155
39
5
4
4
1
1
1
1
1
Host
Mouse
133
94
21
20
14
2
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
25
14
11
8
8
7
7
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
180
170
101
45
44
38
29
21
8
4
4
3
2
1
1
1
Options
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Catalog No. ABIN967446
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
1 ABIN4900705 FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
1 ABIN4900709 FACS IP Hamster IgG Log in to see 145-2C11 8
1 ABIN4900710 FACS IP Hamster IgG Log in to see 145-2C11 8
1 ABIN4900706 FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
1 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
1 ABIN373196 IHC (p) IP Rabbit Log in to see Polyclonal
1 ABIN4291755 CyTOF FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968
1 ABIN4900655 FACS ICC IHC IP Biotin Rat IgG2b Log in to see 17A2
1 ABIN211899 FACS IHC (fro) IP ELISA WB Mouse IgG2a Cytoplasmic Domain Log in to see G3
1 ABIN4291765 FACS ICC IF IP WB PerCP Armenian Hamster IgG Log in to see H146-968
1 ABIN1820406 IP IHC WB Rabbit AA 36-54 Log in to see Polyclonal
1 ABIN2739151 ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see APA1-1
1 ABIN480375 Func IP ELISA WB Mouse IgG1 Cytoplasmic Domain Log in to see
1 ABIN600630 IHC (fro) FACS IP ELISA WB Mouse IgG2a Cytoplasmic Domain Log in to see

Top referenced anti-CD247 antibody for Immunoprecipitation

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 22 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 103 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 104 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope C-Term
(34), (22), (16), (16), (14), (14), (12), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(244), (155), (39), (5), (4), (4), (1), (1), (1), (1), (1)
Host Mouse
(133), (94), (21), (20), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(25), (14), (11), (8), (8), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(180), (170), (101), (45), (44), (38), (29), (21), (8), (4), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CD3zeta C-terminus synthetic peptide
Clone 1zeta3A1
Isotype IgG2b

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain
Molecular Weight 32 kDa
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN967446) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD247 Antibody (ABIN967446)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, 1993

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (C-Term) (ABIN967446) Western blot analysis of CD3zeta. Lysate from mouse thymus was probed with CD3zeta (c...
 image for anti-CD247 antibody (CD247 Molecule) (C-Term) (ABIN967446) anti-CD247 Molecule (CD247) (C-Term) antibody (Image 2)