anti-Mouse (Murine) CD247 antibody for Immunoprecipitation

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • im:7156386
  • LOC100136514
  • CD3Z
  • LOC100136549
  • LOC100305023
  • CD247
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • CD3-ZETA
  • CD3H
  • CD3Q
  • T3Z
  • TCRZ
  • TCRzeta
  • CD247 antigen like
  • CD3zeta-2
  • CD3zeta-1
  • CD3 zeta
  • CD247 molecule
  • CD247 antigen
  • Cd247 molecule
  • cd247l
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • cd247
  • Cd247
Reactivity
Human, Mouse (Murine)
257
162
40
5
4
4
1
1
1
1
1
Host
Mouse
139
101
21
21
14
3
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
26
14
11
8
8
7
7
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
194
176
106
44
44
38
29
24
7
5
4
4
3
1
1
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Catalog No. ABIN967305
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900710 CyTOF FACS IP Hamster IgG Log in to see 145-2C11 8
1 ABIN4900706 CyTOF FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
1 ABIN4900705 CyTOF FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
1 ABIN4900709 CyTOF FACS IP Hamster IgG Log in to see 145-2C11 8
1 ABIN967446 IP WB Mouse IgG2b C-Term Log in to see 1zeta3A1 1
1 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
1 ABIN211899 FACS IHC (fro) IP ELISA WB Mouse IgG2a Cytoplasmic Domain Log in to see G3
1 ABIN4900655 FACS ICC IHC IP Biotin Rat IgG2b Log in to see 17A2
1 ABIN4291755 CyTOF FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968
1 ABIN1820406 IP IHC WB Rabbit AA 36-54 Log in to see Polyclonal
1 ABIN5552374 IHC (p) IP Rabbit Log in to see Polyclonal
1 ABIN2739151 ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see APA1-1
1 ABIN4291765 FACS ICC IF IP WB PerCP Armenian Hamster IgG Log in to see H146-968
1 ABIN480375 Func IP ELISA WB Mouse IgG1 Cytoplasmic Domain Log in to see
1 ABIN600630 IHC (fro) FACS IP ELISA WB Mouse IgG2a Cytoplasmic Domain Log in to see

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human, Mouse (Murine)
(257), (162), (40), (5), (4), (4), (1), (1), (1), (1), (1)
Host Mouse
(139), (101), (21), (21), (14), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(26), (14), (11), (8), (8), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(194), (176), (106), (44), (44), (38), (29), (24), (7), (5), (4), (4), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN967305) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CD3zeta synthetic peptide
Clone 8D3
Isotype IgG1

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 antibody (ABIN967305) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, gamma (gamma), delta (delta), epsilon (epsilon), zeta (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32 (dimer), and 21 kDa respectively. The 8D3 antibody recognizes the zeta chain of the human and mouse T cell antigen receptor-associated CD3 complex. It reacts with 16 kDa unphosphorylated monomers and 32 kDa unphosphorylated dimers. It does not appear to recognize 21 kDa phosphorylated monomers or 42 kDa phosphorylated dimers. A synthetic peptide corresponding to a region of human CD3zeta was used as immunogen.
Synonyms: CD3zeta chain
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 antibody (ABIN967305) Images back to top
Application Notes Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. 51-16526N, store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 antibody (ABIN967305) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CD247 antibody (ABIN967305)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, 1993

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN967305) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 Molecule (CD247) antibody (ABIN967305) Western blot analysis of CD3zeta. Lysate from Jurkat cells was probed with anti-CD3ze...