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anti-Mouse (Murine) CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Epitope
C-Term
34
28
23
22
22
14
14
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
269
120
29
4
2
2
1
1
Host
Mouse
156
99
14
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
29
21
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
198
137
107
57
47
44
34
21
12
8
3
2
1
1
Supplier
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Catalog No. ABIN967446
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
0.13476625 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
0.13476625 ABIN1531309 ELISA WB Rabbit IgG pTyr142 Log in to see Polyclonal
0.13476625 ABIN1532328 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2749060 ICC FACS WB Mouse IgG1 pTyr72 Log in to see EM-26 1
0.13476625 ABIN2705768 WB Rabbit Center Log in to see Polyclonal
0.13476625 ABIN1871602 IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2163416 ELISA WB Rabbit IgG Tyr142 Log in to see Polyclonal
0.13476625 ABIN2163417 ELISA WB Rabbit IgG Tyr42 Log in to see Polyclonal
0.13476625 ABIN951241 EIA FACS IF WB Hamster IgG Log in to see H146-968
0.13476625 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17
0.13476625 ABIN3022813 IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2749059 FACS WB Mouse IgG1 pTyr111, pTyr123 Log in to see EM-55
0.13476625 ABIN2749058 FACS WB Mouse IgG1 pTyr142 Log in to see EM-54
0.13476625 ABIN2424703 WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2405002 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN1847268 ELISA WB Rabbit C-Term, pTyr142 Log in to see Polyclonal
0.0011760078 ABIN272006 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011760078 ABIN118361 EIA IHC (fro) IP WB Mouse IgG2a Log in to see G3 1
0.0011760078 ABIN501184 EIA FACS IHC (fro) IP WB Mouse IgG2a Cytoplasmic Domain Log in to see G3 1

Top referenced anti-CD247 antibody for Western Blotting

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 21 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 108 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope C-Term
(34), (28), (23), (22), (22), (14), (14), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(269), (120), (29), (4), (2), (2), (1), (1)
Host Mouse
(156), (99), (14), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(29), (21), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(198), (137), (107), (57), (47), (44), (34), (21), (12), (8), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CD3zeta C-terminus synthetic peptide
Clone 1zeta3A1
Isotype IgG2b

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain
Molecular Weight 32 kDa
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN967446) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD247 Antibody (ABIN967446)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, 1993

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (C-Term) (ABIN967446) Western blot analysis of CD3zeta. Lysate from mouse thymus was probed with CD3zeta (c...
 image for anti-CD247 antibody (CD247 Molecule) (C-Term) (ABIN967446) anti-CD247 Molecule (CD247) (C-Term) antibody (Image 2)