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|Application / Reactivity||Mouse (Murine)|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||74 Antibodies|
|Immunocytochemistry (ICC)||19 Antibodies|
|Immunofluorescence (IF)||19 Antibodies|
|Immunohistochemistry (IHC)||9 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||95 Antibodies|
|Antigen||CD247 Molecule (CD247) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD247 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CD247 AntibodyTarget Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse CD3zeta C-terminus synthetic peptide|
Target Details CD247Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
|Alternative Name||CD247 (CD247 Antibody Abstract)|
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain
|Molecular Weight||32 kDa|
Application DetailsProduct Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN967446) Images back to top
|Application Notes||Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10  cells). Immunoprecipitation application is not routinely tested, only tested during development.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN967446) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-CD247 Antibody (ABIN967446)Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
|Product cited in:||
Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, 1993
ImagesProduct Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967446) back to top