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anti-Human CD28 antibody for Biochemical Assay

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human
314
306
102
38
26
12
6
5
5
2
2
2
1
1
1
1
Host
Mouse
359
189
77
42
25
20
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to PE-Cy5
106
90
66
41
10
8
7
7
7
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Biochemical Assay (BCA)
541
174
114
95
92
43
41
34
27
22
13
8
4
3
3
3
3
2
2
2
1
1
1
1
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Catalog No. ABIN2658953
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088366197 ABIN2664495 BCA Mouse IgG1 kappa Log in to see CD28-2 14
0.00088366197 ABIN1177331 BCA FACS Mouse IgG1, kappa Log in to see CD28-2

Top referenced anti-CD28 antibody for Biochemical Assay

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 188 Antibodies
Functional Studies (Func) 44 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(314), (306), (102), (38), (26), (12), (6), (5), (5), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(359), (189), (77), (42), (25), (20), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to PE-Cy5
(106), (90), (66), (41), (10), (8), (7), (7), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Biochemical Assay (BCA)
(541), (174), (114), (95), (92), (43), (41), (34), (27), (22), (13), (8), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2658953) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Capuchin Monkey, Sooty mangabey (Cercocebus atys), Squirrel Monkey (Saimiri spec.)
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Clone CD28-2
Isotype IgG1 kappa

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN2658953) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN2658953) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN2658953) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD28 Antibody (ABIN2658953)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004

Rivollier, Mazzorana, Tebib, Piperno, Aitsiselmi, Rabourdin-Combe, Jurdic, Servet-Delprat: "Immature dendritic cell transdifferentiation into osteoclasts: a novel pathway sustained by the rheumatoid arthritis microenvironment." in: Blood, Vol. 104, Issue 13, pp. 4029-37, 2004

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994

Linsley, Ledbetter: "The role of the CD28 receptor during T cell responses to antigen." in: Annual review of immunology, Vol. 11, pp. 191-212, 1993

Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2658953) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (PE-Cy5) (ABIN2658953) anti-CD28 (CD28) antibody (PE-Cy5)