anti-Human CD28 antibody for Cell Culture

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 1-152
17
16
15
12
11
11
11
10
8
6
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Human
495
410
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Mouse
526
199
92
70
40
29
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
112
96
74
37
21
20
19
19
18
17
17
13
13
13
13
12
12
12
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Cell Culture (CC), ELISA
780
211
185
160
142
141
133
129
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN1998805
$ 218.90
Plus shipping costs $45.00

General

Antigen CD28 Antibodies
Epitope AA 1-152
(17), (16), (15), (12), (11), (11), (11), (10), (8), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Human
(495), (410), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(526), (199), (92), (70), (40), (29), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(112), (96), (74), (37), (21), (20), (19), (19), (18), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Cell Culture (CC), ELISA
(780), (211), (185), (160), (142), (141), (133), (129), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Human CD28
No cross-reactivity in ELISA with
Mouse CD28
Human CTLA4
Mouse CTLA4
Human B7-1
Human B7-2
Mouse B7-2
Human β-NGF
Human cell lysate (293 cell line)
No Cross-Reactivity Human, Mouse (Murine)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD28 (rh CD28, P10747-1, Met 1-Pro 152,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human CD28 protein
Clone 9H6E2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background T-cell-specific surface glycoprotein CD28, also known as TP44 and CD28, is a single-pass type I membrane protein which contains oneIg-like V-type (immunoglobulin-like) domain. CD28 / TP44 is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 / TP44 is expressed in T-cells and plasma cells, but not in less mature B-cells. CD28 / TP44 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. CD28 / TP44 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. CD28 / TP44 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
UniProt P10747-1
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD28. The detection limit for Human CD28 is approximately 0.039 ng/well.
ELISA(Cap): 0.5-4 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months