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anti-Human CD28 antibody for Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human
441
376
106
71
43
33
26
26
23
23
12
6
2
1
1
1
1
Host
Rat
512
202
77
70
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to PE
119
99
78
41
18
17
15
14
13
12
11
10
9
9
9
9
9
9
8
8
8
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
746
274
176
135
120
119
117
114
86
63
22
13
8
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN118584
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29105452 ABIN1106185 FACS PE Mouse IgG2a Log in to see B-T3 1
0.29105452 ABIN1106183 IHC (fro) FACS Mouse IgG2a Log in to see B-T3 1
0.29105452 ABIN1106184 FACS FITC Mouse IgG2a Log in to see B-T3 1
0.29105452 ABIN1106181 Func IHC (fro) FACS Mouse IgG2a Log in to see B-T3 4
0.29105452 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
0.29105452 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
0.29105452 ABIN2658047 FACS IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN2661032 FACS Biotin Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN2659701 FACS PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN400889 FACS APC Mouse IgG1 Log in to see CD28-2
0.29105452 ABIN400893 FACS PE Mouse IgG1 Log in to see CD28-2
0.29105452 ABIN400892 FACS FITC Mouse IgG1 Log in to see CD28-2
0.29105452 ABIN2657374 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN2665974 FACS Mouse IgG1 kappa Log in to see CD28-2 8
0.29105452 ABIN2660336 FACS CyTOF PerCP-Cy5.5 Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN2661638 FACS ICFC FITC Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN2658579 FACS APC Mouse IgG1 kappa Log in to see CD28-2 9
0.29105452 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
0.29105452 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
0.29105452 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6

Top referenced anti-CD28 antibody for Flow Cytometry

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 101 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 319 Antibodies
Functional Studies (Func) 71 Antibodies
Immunocytochemistry (ICC) 106 Antibodies
Immunofluorescence (IF) 114 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 133 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 115 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(441), (376), (106), (71), (43), (33), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Rat
(512), (202), (77), (70), (28), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to PE
(119), (99), (78), (41), (18), (17), (15), (14), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(746), (274), (176), (135), (120), (119), (117), (114), (86), (63), (22), (13), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN118584) Images
Specificity This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
Purification Affinity chromatography on Protein G.
Immunogen Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Clone YTH913-12
Isotype IgG2b

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN118584) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN118584) Images back to top
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN118584) Images back to top
Reconstitution Restore with distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

References for anti-CD28 Antibody (ABIN118584)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN118584) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (PE) (ABIN118584) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE (ABIN1185...