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anti-Human CD28 antibody for Immunofluorescence

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 182-208, C-Term
20
16
15
14
11
11
10
9
6
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
457
389
102
71
44
33
26
26
24
24
12
6
1
1
1
1
1
Host
Rabbit
519
201
79
71
40
27
12
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
119
99
78
38
20
19
16
16
15
14
13
11
11
11
11
10
10
10
10
7
6
6
5
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
762
212
182
142
141
134
130
114
97
57
22
13
12
8
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.40629 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
1 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN2665915 FACS IF Mouse IgG1 kappa Log in to see CD28-2 10
1 ABIN2665916 FACS IF Mouse IgG1 kappa Log in to see CD28-2 8
1 ABIN1814155 IF WB Rabbit AA 188-217 Log in to see Polyclonal
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
1 ABIN2224809 FACS IF IP IHC Mouse IgG1 kappa Log in to see C28-75
1 ABIN2224798 FACS IF IP IHC Mouse IgG1 kappa Log in to see CB28
1 ABIN2224846 FACS IF IP IHC Mouse IgG1 kappa Log in to see C28-77
1 ABIN4957796 FACS ICC IF DyLight 650 Mouse IgG1 Log in to see C28-1636
1 ABIN4957798 FACS ICC IF DyLight 680 Mouse IgG1 Log in to see C28-1636
1 ABIN4957803 FACS ICC IF DyLight 350 Mouse IgG1 Log in to see C28-1636
1 ABIN4957802 FACS ICC IF DyLight 550 Mouse IgG1 Log in to see C28-1636
1 ABIN4957804 FACS ICC IF DyLight 405 Mouse IgG1 Log in to see C28-1636
1 ABIN4957805 FACS ICC IF DyLight 405LS Mouse IgG1 Log in to see C28-1636
1 ABIN4957799 FACS ICC IF DyLight 755 Mouse IgG1 Log in to see C28-1636
1 ABIN4957790 FACS ICC IF Alexa Fluor 405 Mouse IgG1 Log in to see C28-1636
1 ABIN4957791 FACS ICC IF Alexa Fluor 488 Mouse IgG1 Log in to see C28-1636

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 126 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 329 Antibodies
Functional Studies (Func) 52 Antibodies
Immunocytochemistry (ICC) 112 Antibodies
Immunofluorescence (IF) 123 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 123 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 54 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(20), (16), (15), (14), (11), (11), (10), (9), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(457), (389), (102), (71), (44), (33), (26), (26), (24), (24), (12), (6), (1), (1), (1), (1), (1)
Host Rabbit
(519), (201), (79), (71), (40), (27), (12)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(119), (99), (78), (38), (20), (19), (16), (16), (15), (14), (13), (11), (11), (11), (11), (10), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(762), (212), (182), (142), (141), (134), (130), (114), (97), (57), (22), (13), (12), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 Antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...