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anti-Human CD28 antibody for Immunofluorescence

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Epitope
AA 182-208, C-Term
20
16
15
14
11
11
10
8
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
441
363
106
69
44
31
26
23
23
23
12
6
2
1
1
1
1
Host
Rabbit
512
203
76
66
25
23
7
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
115
96
75
41
17
17
15
13
12
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
734
265
178
131
121
118
116
111
86
66
22
13
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010826335 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
0.0010826335 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
0.0010826335 ABIN2665916 FACS IF Mouse IgG1 kappa Log in to see CD28-2 8
0.0010826335 ABIN2665915 FACS IF Mouse IgG1 kappa Log in to see CD28-2 10
0.0010826335 ABIN1814155 IF WB Rabbit AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN2224798 FACS IF IP IHC Mouse IgG1 kappa Log in to see CB28
0.0010826335 ABIN2224846 FACS IF IP IHC Mouse IgG1 kappa Log in to see C28-77
0.0010826335 ABIN2224809 FACS IF IP IHC Mouse IgG1 kappa Log in to see C28-75
0.0010826335 ABIN2224782 IF ELISA WB PE Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN2224780 IF ELISA WB FITC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN2224804 IF ELISA WB Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN2224828 IF ELISA WB APC Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN4291200 FACS Func ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
0.0010826335 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
0.0010826335 ABIN2224779 IF ELISA WB Biotin Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN2224827 IF ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 188-217 Log in to see Polyclonal
0.0010826335 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
0.0010826335 ABIN3026533 FACS IF IHC Mouse IgG1, kappa Log in to see C28-77
0.0010826335 ABIN3026535 Func FACS IF Mouse IgG1, kappa Log in to see CB28
0.0010826335 ABIN4291247 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 102 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 323 Antibodies
Functional Studies (Func) 68 Antibodies
Immunocytochemistry (ICC) 106 Antibodies
Immunofluorescence (IF) 118 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 139 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 115 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(20), (16), (15), (14), (11), (11), (10), (8), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(441), (363), (106), (69), (44), (31), (26), (23), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Rabbit
(512), (203), (76), (66), (25), (23), (7)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(115), (96), (75), (41), (17), (17), (15), (13), (12), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(734), (265), (178), (131), (121), (118), (116), (111), (86), (66), (22), (13), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 Antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...