anti-Human CD28 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Reactivity
Human
495
414
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Rat
527
203
92
69
40
29
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
113
96
74
56
21
20
20
19
17
17
13
13
13
13
12
12
12
8
8
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
787
211
184
160
143
141
133
129
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN118583
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106183 IHC (fro) FACS Mouse IgG2a Log in to see B-T3 1
1 ABIN1106181 Func IHC (fro) FACS Mouse IgG2a Log in to see B-T3 4
1 ABIN118582 Func FACS IHC (fro) Rat IgG2b Log in to see YTH913-12 1
1 ABIN319709 FACS IHC (fro) Rat IgG2b Log in to see YTH913-12 1
1 ABIN319708 FACS IHC (fro) Rat IgG2b Log in to see YTH913-12 1
1 ABIN610468 IHC (fro) FACS Mouse IgG2a Log in to see 204-12 2
1 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN255869 FACS IHC (fro) IHC Rat IgG2b Log in to see YTH913-12
1 ABIN2689148 IHC (fro) FACS Mouse IgG1, kappa Log in to see CD28-2 1
1 ABIN205423 FACS IHC (fro) IP Mouse IgG1 Log in to see CD28-2
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN600765 IHC (fro) FACS Mouse IgG2a Log in to see 204-12
1 ABIN203924 FACS IHC (fro) IP Mouse IgG1, kappa Log in to see
1 ABIN600764 IHC (fro) FACS Rat IgG2b Log in to see
1 ABIN1106182 IHC (fro) Mouse IgG2a Log in to see 204-12
1 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN445850 CyTOF FACS IHC IHC (fro) Mouse IgG2a Log in to see 204-12
1 ABIN2479697 IHC (fro) FACS Rat IgG2b Log in to see YTH913-12 3
1 ABIN2479698 IHC (fro) FACS Rat IgG2b Log in to see YTH913-12 3

General

Antigen CD28 Antibodies
Reactivity Human
(495), (414), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rat
(527), (203), (92), (69), (40), (29), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(113), (96), (74), (56), (21), (20), (20), (19), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(787), (211), (184), (160), (143), (141), (133), (129), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN118583) Images
Specificity This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.(2)
Purification Affinity chromatography on Protein A.
Immunogen Human peripheral blood T-cells.
Clone YTH913-12
Isotype IgG2b

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN118583) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN118583) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells or 100 μL whole blood). Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN118583) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD28 antibody (ABIN118583)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN118583) back to top
Supplier Images
 image for anti-CD28 (CD28) antibody (ABIN118583) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28 (ABIN118583).