This CD28 antibody is un-conjugated
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Species reactivity (tested):Mouse.
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Peptide with sequence from the internal region of the protein sequence according to NP_031668.3.
CD28 Antibody Abstract)
CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3. CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Optimal working dilution should be determined by the investigator.
For Research Use only
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Avoid repeated freezing and thawing.
4 °C/-20 °C
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
AP23670PU-N CD28 antibody staining of Mouse Thymus lysate at 0.3 µg/ml (35 µg protein...