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anti-Mouse (Murine) CD28 antibody for Enzyme Immunoassay

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
Internal Region
16
15
13
11
11
11
11
10
8
7
6
4
4
4
3
3
3
3
1
1
1
1
1
1
Reactivity
Mouse (Murine)
466
383
102
70
50
34
27
26
25
22
15
6
3
3
3
2
1
1
1
Host
Goat
500
190
89
70
40
25
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
109
91
72
36
20
18
18
17
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
746
208
184
145
140
134
129
127
88
72
26
13
13
9
8
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN615889
$ 379.50
Plus shipping costs $45.00

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General

Antigen CD28 Antibodies
Epitope Internal Region
(16), (15), (13), (11), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(466), (383), (102), (70), (50), (34), (27), (26), (25), (22), (15), (6), (3), (3), (3), (2), (1), (1), (1)
Host Goat
(500), (190), (89), (70), (40), (25), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(109), (91), (72), (36), (20), (18), (18), (17), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(746), (208), (184), (145), (140), (134), (129), (127), (88), (72), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Sequence KEKHLCHTQS SPK
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_031668.3.

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3. CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Molecular Weight 25.2kDa (NP_031668.3).
NCBI Accession NP_031668
UniProt P31041
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (Internal Region) (ABIN615889) AP23670PU-N CD28 antibody staining of Mouse Thymus lysate at 0.3 µg/ml (35 µg protein...