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anti-Mouse (Murine) CD28 antibody for ELISA (Detection)

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Mouse (Murine)
467
406
106
72
44
34
26
26
24
24
12
6
1
1
1
1
Host
Mouse
522
214
84
72
40
28
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
120
99
78
38
21
20
17
16
16
15
14
12
12
12
12
11
10
10
10
7
6
6
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection)), Immunofluorescence (IF)
784
213
181
141
141
135
134
126
97
67
26
13
13
8
6
4
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN2664499
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Top referenced anti-CD28 antibody for ELISA (Detection)

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Application / Reactivity Mouse (Murine)
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General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(467), (406), (106), (72), (44), (34), (26), (26), (24), (24), (12), (6), (1), (1), (1), (1)
Host Mouse
(522), (214), (84), (72), (40), (28), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(120), (99), (78), (38), (21), (20), (17), (16), (16), (15), (14), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection)), Immunofluorescence (IF)
(784), (213), (181), (141), (141), (135), (134), (126), (97), (67), (26), (13), (13), (8), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2664499) Images
Purification The antibody was purified by affinity chromatography.
Clone E18
Isotype IgG2b kappa

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN2664499) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN2664499) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN2664499) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD28 Antibody (ABIN2664499)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Dennehy, Elias, Zeder-Lutz, Ding, Altschuh, Lühder, Hünig: "Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5725-9, 2006

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2664499) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN2664499) anti-CD28 (CD28) antibody